Detailed information NTG0369686

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D069_RS12425
• Genome accession: NZ_CP041143
• Coordinates: 2534776..2535390 (-)
• Length: 204 a.a.
• NCBI ID: WP_105937505.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain UCMB5007
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2529776..2540390

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D069_RS12395	rpsT	2530079..2530345 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
D069_RS12400 (D069_2311)	holA	2530378..2531403 (-)	1026	Protein_2376	DNA polymerase III subunit delta	-
D069_RS12405	-	2531443..2531594 (-)	152	Protein_2377	hypothetical protein	-
D069_RS12410	-	2531635..2531769 (+)	135	WP_003152870.1	YqzM family protein	-
D069_RS12415 (D069_2312)	comEC	2531788..2534139 (-)	2352	WP_105937506.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D069_RS12420 (D069_2313)	-	2534140..2534709 (-)	570	WP_003152868.1	ComE operon protein 2	-
D069_RS12425 (D069_2314)	comEA	2534776..2535390 (-)	615	WP_105937505.1	helix-hairpin-helix domain-containing protein	Machinery gene
D069_RS12430 (D069_2315)	comER	2535449..2536270 (+)	822	WP_012118027.1	late competence protein ComER	-
D069_RS12435 (D069_2316)	-	2536339..2537076 (-)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
D069_RS12440 (D069_2317)	rsfS	2537073..2537429 (-)	357	WP_007408260.1	ribosome silencing factor	-
D069_RS12445 (D069_2318)	yqeK	2537447..2538007 (-)	561	WP_140957459.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D069_RS12450 (D069_2319)	-	2537997..2538566 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
D069_RS12455 (D069_2320)	yhbY	2538577..2538867 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
D069_RS12460 (D069_2321)	aroE	2538861..2539703 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22206.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=369686 D069_RS12425 WP_105937505.1 2534776..2535390(-) (comEA) [Bacillus velezensis strain UCMB5007]
MTKAMMTERLRRNLKILGACLVAGAVFICILLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTVEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=369686 D069_RS12425 WP_105937505.1 2534776..2535390(-) (comEA) [Bacillus velezensis strain UCMB5007]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTTGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGTTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATAACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.592	93.627	0.539
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363
comEA	Latilactobacillus sakei subsp. sakei 23K	32.314	100	0.363