Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   D069_RS12000 Genome accession   NZ_CP041143
Coordinates   2458260..2459330 (-) Length   356 a.a.
NCBI ID   WP_007612574.1    Uniprot ID   -
Organism   Bacillus velezensis strain UCMB5007     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2453260..2464330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D069_RS11950 (D069_2225) sipW 2453319..2453903 (-) 585 WP_015240205.1 signal peptidase I SipW -
  D069_RS11955 (D069_2226) tapA 2453875..2454546 (-) 672 WP_044802562.1 amyloid fiber anchoring/assembly protein TapA -
  D069_RS11960 (D069_2227) - 2454805..2455134 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  D069_RS11965 (D069_2228) - 2455175..2455354 (-) 180 WP_022552966.1 YqzE family protein -
  D069_RS11970 (D069_2229) comGG 2455411..2455788 (-) 378 WP_038459177.1 competence type IV pilus minor pilin ComGG Machinery gene
  D069_RS11975 (D069_2230) comGF 2455789..2456289 (-) 501 WP_228842201.1 competence type IV pilus minor pilin ComGF -
  D069_RS11980 (D069_2231) comGE 2456198..2456512 (-) 315 WP_105937524.1 competence type IV pilus minor pilin ComGE -
  D069_RS11985 (D069_2232) comGD 2456496..2456933 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  D069_RS11990 (D069_2233) comGC 2456923..2457231 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  D069_RS11995 (D069_2234) comGB 2457236..2458273 (-) 1038 WP_105937523.1 competence type IV pilus assembly protein ComGB Machinery gene
  D069_RS12000 (D069_2235) comGA 2458260..2459330 (-) 1071 WP_007612574.1 competence type IV pilus ATPase ComGA Machinery gene
  D069_RS12005 (D069_2236) - 2459528..2460478 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  D069_RS12010 (D069_2237) - 2460624..2461925 (+) 1302 WP_105937521.1 hemolysin family protein -
  D069_RS12015 (D069_2238) - 2461963..2462793 (-) 831 WP_029326073.1 STAS domain-containing protein -
  D069_RS12025 (D069_2240) - 2462999..2463379 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D069_RS12030 (D069_2241) - 2463616..2463861 (+) 246 WP_105937520.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.84 Da        Isoelectric Point: 9.3437

>NTDB_id=369682 D069_RS12000 WP_007612574.1 2458260..2459330(-) (comGA) [Bacillus velezensis strain UCMB5007]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRETRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=369682 D069_RS12000 WP_007612574.1 2458260..2459330(-) (comGA) [Bacillus velezensis strain UCMB5007]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTCGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGGAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

38.177

98.596

0.376

Multiple sequence alignment