Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FY142_RS05190 Genome accession   NZ_CP048528
Coordinates   1053168..1054784 (+) Length   538 a.a.
NCBI ID   WP_099085445.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain Sta003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048168..1059784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY142_RS05165 (FY142_05130) dinR/lexA 1048590..1049312 (-) 723 WP_099085450.1 transcriptional repressor LexA Regulator
  FY142_RS05170 (FY142_05135) - 1050110..1051126 (+) 1017 WP_144030928.1 PilN domain-containing protein -
  FY142_RS05175 (FY142_05140) gspM 1051123..1051719 (+) 597 WP_099085448.1 type II secretion system protein GspM -
  FY142_RS05180 (FY142_05145) - 1051716..1052285 (+) 570 WP_099085447.1 hypothetical protein -
  FY142_RS05185 (FY142_05150) - 1052275..1053171 (+) 897 WP_111817215.1 hypothetical protein -
  FY142_RS05190 (FY142_05155) pilF 1053168..1054784 (+) 1617 WP_099085445.1 GspE/PulE family protein Machinery gene
  FY142_RS05195 (FY142_05160) - 1054786..1055997 (+) 1212 WP_099085444.1 type II secretion system F family protein -
  FY142_RS05200 (FY142_05165) - 1055994..1056479 (+) 486 WP_099085443.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY142_RS05205 (FY142_05170) - 1056472..1056840 (+) 369 WP_099085442.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY142_RS05210 (FY142_05175) - 1056837..1057481 (+) 645 WP_099085441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY142_RS05215 (FY142_05180) gspG 1057517..1057966 (-) 450 WP_099085440.1 type II secretion system major pseudopilin GspG -
  FY142_RS05220 (FY142_05185) - 1057974..1058426 (-) 453 WP_162686857.1 prepilin peptidase -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 58673.17 Da        Isoelectric Point: 5.4214

>NTDB_id=369240 FY142_RS05190 WP_099085445.1 1053168..1054784(+) (pilF) [Agrobacterium tumefaciens strain Sta003]
MTTIFPTSDEFLAHLGTSSRLTPEALQRLSSAWRATGQPIDVVMRELGLMPEAELAQAIADFCMIGCAQSVFEADSEMLE
RMGTDFAARNALLPITGEKGALSLIVADPFSRPAIEAIEFLIGGRLPLIVAPRSTIEEFFRVRKAVRDDGDFSGSHDVAG
EEDVDRLLDIAKEAPVIRFVSRIIQRAVDGKATDIHIEPFEDIVRIRLRTDGILQTLETVPRSMAAGITSRIKILSRLNI
AERRLPQDGRLRASVRGQEIDFRVSVTPSVHGETVVMRILDRSNVRLELGALGFDEQATAAIAAITRKPNGIFLVTGPTG
SGKTTTLYSILNDINDPRWKFFTVEDPVEYRMKGITQLQVDPGIGLTFASALRSVLRQDPDGILIGEIRDEETARIAVQA
ALTGHLVLSTLHTNSAAGAISRLRNMGIESYLLAATLRGIIGQRLVRRVCEECRHHDSETMCAVCGGSGLAGRQAVYEIM
EITDALRDSISRKDDENALEMAAQQVGMRSMREHAHGLVAAGVTTSGEVSRVIDLQGA

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=369240 FY142_RS05190 WP_099085445.1 1053168..1054784(+) (pilF) [Agrobacterium tumefaciens strain Sta003]
GTGACCACCATATTTCCGACTTCGGATGAGTTTCTCGCGCATCTCGGCACCAGCTCAAGGTTGACACCCGAGGCGCTTCA
ACGGCTGTCCTCCGCCTGGCGCGCCACAGGTCAACCGATCGATGTTGTCATGCGTGAGCTCGGGCTGATGCCGGAAGCCG
AACTGGCGCAGGCGATTGCAGATTTCTGCATGATCGGCTGCGCGCAATCGGTTTTCGAGGCAGACAGTGAGATGCTGGAA
CGGATGGGTACGGATTTTGCAGCGAGGAATGCACTTCTGCCCATAACAGGAGAGAAAGGAGCGCTCTCCCTGATCGTTGC
GGACCCTTTTAGCCGCCCGGCCATAGAAGCGATCGAGTTTCTGATCGGGGGGCGTCTTCCGCTCATTGTCGCGCCGAGAT
CGACGATTGAGGAGTTTTTTCGGGTTCGCAAGGCCGTGCGGGACGATGGTGATTTTTCCGGGTCCCATGACGTTGCAGGC
GAGGAGGACGTTGATCGGTTGCTCGACATTGCGAAAGAGGCGCCGGTCATTCGCTTCGTATCCAGAATCATTCAGCGGGC
CGTCGATGGCAAAGCGACGGATATTCATATCGAGCCTTTTGAGGATATTGTGAGAATTCGTTTGCGGACAGACGGCATTC
TTCAAACCCTGGAGACCGTGCCGAGATCGATGGCGGCCGGTATAACGAGCCGGATCAAGATATTGTCGCGGCTCAATATT
GCGGAGCGACGCTTACCCCAGGACGGGCGGCTGCGGGCATCCGTTCGCGGGCAGGAGATCGATTTTCGAGTCTCCGTGAC
GCCCAGCGTCCACGGCGAAACCGTTGTCATGCGAATTCTCGATCGCTCGAATGTGCGCCTCGAATTGGGTGCCCTTGGAT
TTGATGAGCAGGCGACCGCGGCGATCGCGGCCATTACGCGCAAACCGAACGGGATATTTCTCGTCACCGGGCCAACGGGT
TCCGGCAAGACGACGACACTCTACTCCATTCTAAACGACATCAACGATCCGAGGTGGAAGTTCTTCACGGTTGAAGATCC
CGTCGAGTATCGGATGAAGGGAATTACCCAGTTGCAGGTCGATCCGGGGATTGGTCTGACCTTCGCCTCAGCCCTGCGTT
CAGTTCTCCGGCAGGACCCTGACGGCATCCTGATTGGGGAAATCCGAGACGAGGAGACGGCCAGAATCGCCGTGCAGGCG
GCGCTGACCGGTCATCTTGTTCTATCGACGCTGCATACCAACAGCGCCGCCGGGGCTATCAGCCGGCTTCGCAACATGGG
GATCGAAAGCTACCTTCTTGCCGCAACTTTGCGGGGGATCATCGGCCAGAGGCTGGTTCGCAGGGTGTGCGAGGAATGCC
GGCATCACGACAGCGAGACGATGTGTGCCGTTTGTGGCGGCTCCGGTTTGGCCGGACGGCAAGCCGTCTATGAGATCATG
GAAATCACGGATGCGTTGCGAGATTCGATTTCCAGAAAAGACGACGAAAATGCCCTCGAAATGGCGGCACAGCAGGTGGG
TATGCGAAGCATGCGGGAGCATGCCCATGGGCTTGTTGCTGCCGGAGTGACGACCTCGGGCGAGGTTTCGCGCGTCATCG
ACCTTCAGGGAGCCTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(183-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

37.9

100

0.396
  pilB Vibrio parahaemolyticus RIMD 2210633

37.454

100

0.377
  pilB Acinetobacter baumannii D1279779

36.447

100

0.37
  pilB Legionella pneumophila strain ERS1305867

40.664

89.591

0.364
  pilB Vibrio campbellii strain DS40M4

36.567

99.628

0.364
  pilB Vibrio cholerae strain A1552

36.517

99.257

0.362