Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GZS12_RS03625 Genome accession   NZ_CP048337
Coordinates   764544..765203 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 142     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 759544..770203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS12_RS03595 (GZS12_03595) - 759660..760607 (+) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -
  GZS12_RS03600 (GZS12_03600) - 760604..761491 (+) 888 WP_000614960.1 MurR/RpiR family transcriptional regulator -
  GZS12_RS03605 (GZS12_03605) ygiN 761536..761850 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  GZS12_RS03610 (GZS12_03610) mdaB 761881..762462 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GZS12_RS03615 (GZS12_03615) ygiZ 762820..763152 (+) 333 WP_022646136.1 DUF2645 family protein -
  GZS12_RS03620 (GZS12_03620) qseC 763198..764547 (-) 1350 WP_022646135.1 quorum sensing histidine kinase QseC -
  GZS12_RS03625 (GZS12_03625) ciaR 764544..765203 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GZS12_RS03630 (GZS12_03630) ygiW 765355..765747 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GZS12_RS03635 (GZS12_03635) ygiV 765800..766282 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  GZS12_RS03640 (GZS12_03640) ygiS 766391..767998 (+) 1608 WP_022646134.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=368005 GZS12_RS03625 WP_001221493.1 764544..765203(-) (ciaR) [Escherichia coli strain 142]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=368005 GZS12_RS03625 WP_001221493.1 764544..765203(-) (ciaR) [Escherichia coli strain 142]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment