Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GZS14_RS03545 Genome accession   NZ_CP048326
Coordinates   736079..736738 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 61     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 731079..741738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS14_RS03520 (GZS14_03525) parE 731170..733062 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  GZS14_RS03525 (GZS14_03530) ygiN 733110..733424 (-) 315 WP_074684072.1 putative quinol monooxygenase -
  GZS14_RS03530 (GZS14_03535) mdaB 733455..734036 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GZS14_RS03535 (GZS14_03540) ygiZ 734355..734687 (+) 333 WP_000912120.1 DUF2645 family protein -
  GZS14_RS03540 (GZS14_03545) qseC 734733..736082 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  GZS14_RS03545 (GZS14_03550) ciaR 736079..736738 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  GZS14_RS03550 (GZS14_03555) ygiW 736890..737282 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GZS14_RS03555 (GZS14_03560) ygiV 737335..737817 (+) 483 WP_000183481.1 GyrI-like domain-containing protein -
  GZS14_RS03560 (GZS14_03565) parC 738363..740621 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  GZS14_RS03565 (GZS14_03570) plsC 740854..741591 (+) 738 WP_000965703.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=367845 GZS14_RS03545 WP_001221502.1 736079..736738(-) (ciaR) [Escherichia coli strain 61]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=367845 GZS14_RS03545 WP_001221502.1 736079..736738(-) (ciaR) [Escherichia coli strain 61]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365