Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   GZS16_RS00530 Genome accession   NZ_CP048310
Coordinates   111589..113670 (-) Length   693 a.a.
NCBI ID   WP_000678480.1    Uniprot ID   -
Organism   Escherichia coli strain 32-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 106589..118670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS16_RS00515 (GZS16_00515) yicH 106717..108426 (-) 1710 WP_001333730.1 AsmA family protein -
  GZS16_RS00520 (GZS16_00520) xanP 108547..109938 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  GZS16_RS00525 (GZS16_00525) gltS 110218..111423 (+) 1206 WP_000468833.1 sodium/glutamate symporter -
  GZS16_RS00530 (GZS16_00530) recG 111589..113670 (-) 2082 WP_000678480.1 ATP-dependent DNA helicase RecG Machinery gene
  GZS16_RS00535 (GZS16_00535) trmH 113676..114365 (-) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  GZS16_RS00540 (GZS16_00540) spoT 114372..116480 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  GZS16_RS00545 (GZS16_00545) rpoZ 116499..116774 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  GZS16_RS00550 (GZS16_00550) gmk 116829..117452 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76505.48 Da        Isoelectric Point: 7.5043

>NTDB_id=367670 GZS16_RS00530 WP_000678480.1 111589..113670(-) (recG) [Escherichia coli strain 32-4]
MKGRLLDTVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIKVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=367670 GZS16_RS00530 WP_000678480.1 111589..113670(-) (recG) [Escherichia coli strain 32-4]
ATGAAAGGCCGCCTGTTAGATACTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATTTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGCACGCCAGAATTACAGGAAACGCTCACGCCGGTTTATCCAACAACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCACCGACGCTACAGCTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCACAACCTCAGCATGTTAGCCTTACGT
GCCGGAGCACAGCGTTTTCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCATGATATGGCGCTGGATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCACTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGGTTTGCACCGCTCGGTATCAA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGCAAAGCGCGACTGTCTCAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCAATGACGGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATA
ACTGAAGGTCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAATTGAATGTTGGCCTGGTACACGGGCGGATGAAACCAGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACATCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGCCTGATGATTATCGAAAACCCTGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCTTGATAGAACGCTGGATGCCGGAGACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

DEAD/DEAH box helicase (DEAD)

(292-433)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

39.219

99.711

0.391
  recG/mmsA Streptococcus pneumoniae R36A

39.219

99.711

0.391
  recG Bacillus subtilis subsp. subtilis str. 168

39.21

94.949

0.372