Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   GWK05_RS23505 Genome accession   NZ_CP048107
Coordinates   4926800..4928998 (+) Length   732 a.a.
NCBI ID   WP_022646377.1    Uniprot ID   -
Organism   Escherichia coli strain 201609     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4921800..4933998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK05_RS23495 (GWK05_23460) rhsC 4922041..4926225 (-) 4185 WP_096040764.1 rhs element protein RhsC -
  GWK05_RS23500 (GWK05_23465) rpmE 4926385..4926597 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  GWK05_RS23505 (GWK05_23470) priA 4926800..4928998 (+) 2199 WP_022646377.1 primosomal protein N' Machinery gene
  GWK05_RS23510 (GWK05_23475) cytR 4929154..4930179 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  GWK05_RS23515 (GWK05_23480) ftsN 4930271..4931230 (+) 960 WP_000068834.1 cell division protein FtsN -
  GWK05_RS23520 (GWK05_23485) hslV 4931323..4931853 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  GWK05_RS23525 (GWK05_23490) hslU 4931863..4933194 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81671.00 Da        Isoelectric Point: 9.1493

>NTDB_id=367206 GWK05_RS23505 WP_022646377.1 4926800..4928998(+) (priA) [Escherichia coli strain 201609]
MPVAHIALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=367206 GWK05_RS23505 WP_022646377.1 4926800..4928998(+) (priA) [Escherichia coli strain 201609]
ATGCCCGTTGCCCACATTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCATTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTTAACAGCCTGAAAC
GCTCACCCAAGCAACAACAGGCACTGGCAGCGTTACGGCAAGGCAAAATCTGGCGTGATCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
CACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACAGGTTCCGGTAAAACAGAGGTTTATTTGAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGATAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACATTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAACCG
CCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGTGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGTTGCCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCTTGCGGT
TCCACGCACCTGGTCCCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCACCGTTGTTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGTAAAGGTGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGTGGCGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCTGAACATCCGCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCACTGGCTGAACGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAACTGCGTAATCTCATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCTCCGAAACGTGGTGGTCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCACTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTACTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.64

100

0.449

Multiple sequence alignment