Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GWK05_RS04090 Genome accession   NZ_CP048107
Coordinates   859016..859675 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 201609     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 854016..864675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK05_RS04065 (GWK05_04060) - 854758..855705 (+) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -
  GWK05_RS04070 (GWK05_04065) - 855702..856589 (+) 888 WP_000614945.1 MurR/RpiR family transcriptional regulator -
  GWK05_RS04075 (GWK05_04070) ygiN 856634..856948 (-) 315 WP_000633737.1 putative quinol monooxygenase -
  GWK05_RS04080 (GWK05_04075) mdaB 856979..857560 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GWK05_RS04085 (GWK05_04080) qseC 857670..859019 (-) 1350 WP_000673347.1 quorum sensing histidine kinase QseC -
  GWK05_RS04090 (GWK05_04085) ciaR 859016..859675 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GWK05_RS04095 (GWK05_04090) ygiW 859827..860219 (+) 393 WP_012311800.1 OB fold stress tolerance protein YgiW -
  GWK05_RS04100 (GWK05_04095) ygiV 860272..860754 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  GWK05_RS04105 (GWK05_04100) ygiS 860863..862470 (+) 1608 WP_012311669.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=367148 GWK05_RS04090 WP_001221493.1 859016..859675(-) (ciaR) [Escherichia coli strain 201609]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=367148 GWK05_RS04090 WP_001221493.1 859016..859675(-) (ciaR) [Escherichia coli strain 201609]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTTTTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAGCCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365