Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   GV831_RS10820 Genome accession   NZ_CP047891
Coordinates   2176165..2176611 (+) Length   148 a.a.
NCBI ID   WP_029344525.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain WHH2311     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171165..2181611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV831_RS10780 (GV831_10730) - 2172834..2173070 (+) 237 WP_014570799.1 hypothetical protein -
  GV831_RS10785 (GV831_10735) - 2173083..2173433 (+) 351 WP_014570798.1 hypothetical protein -
  GV831_RS10790 (GV831_10740) - 2173446..2173745 (+) 300 WP_014570797.1 phage holin -
  GV831_RS10795 (GV831_10745) - 2173745..2174524 (+) 780 WP_014570796.1 peptidoglycan amidohydrolase family protein -
  GV831_RS10800 (GV831_10750) - 2174603..2175127 (+) 525 WP_014570795.1 GNAT family N-acetyltransferase -
  GV831_RS10805 (GV831_10755) comGC 2175274..2175543 (+) 270 WP_003129998.1 competence type IV pilus major pilin ComGC Machinery gene
  GV831_RS10810 (GV831_10760) comGD 2175503..2175934 (+) 432 WP_014570794.1 competence type IV pilus minor pilin ComGD Machinery gene
  GV831_RS10815 (GV831_10765) comGE 2175906..2176202 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  GV831_RS10820 (GV831_10770) comGF 2176165..2176611 (+) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  GV831_RS10825 (GV831_10775) comGG 2176650..2176934 (+) 285 WP_003129993.1 competence type IV pilus minor pilin ComGG Machinery gene
  GV831_RS10830 (GV831_10780) - 2177024..2177461 (+) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  GV831_RS10835 (GV831_10785) - 2177458..2178300 (+) 843 WP_003129990.1 metal ABC transporter substrate-binding protein -
  GV831_RS10840 (GV831_10790) - 2178477..2179214 (+) 738 WP_003129989.1 metal ABC transporter ATP-binding protein -
  GV831_RS10845 (GV831_10795) - 2179207..2180016 (+) 810 WP_014570791.1 metal ABC transporter permease -
  GV831_RS10850 (GV831_10800) - 2180055..2180921 (-) 867 WP_003129984.1 RluA family pseudouridine synthase -
  GV831_RS10855 (GV831_10805) - 2181125..2181502 (+) 378 WP_003129983.1 pyridoxamine 5'-phosphate oxidase family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17111.91 Da        Isoelectric Point: 8.6780

>NTDB_id=366440 GV831_RS10820 WP_029344525.1 2176165..2176611(+) (comGF) [Lactococcus lactis subsp. lactis strain WHH2311]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=366440 GV831_RS10820 WP_029344525.1 2176165..2176611(+) (comGF) [Lactococcus lactis subsp. lactis strain WHH2311]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

76.259

93.919

0.716
  comGF Streptococcus mutans UA140

48.227

95.27

0.459
  comGF Streptococcus mutans UA159

47.518

95.27

0.453
  comGF/cglF Streptococcus mitis SK321

43.066

92.568

0.399
  comGF/cglF Streptococcus pneumoniae Rx1

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae D39

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae R6

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae TIGR4

40.426

95.27

0.385
  comGF/cglF Streptococcus mitis NCTC 12261

41.606

92.568

0.385