Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   FGE23_RS17455 Genome accession   NZ_CP040672
Coordinates   3535273..3535887 (-) Length   204 a.a.
NCBI ID   WP_146277193.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain X030     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3530273..3540887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGE23_RS17425 rpsT 3530573..3530839 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  FGE23_RS17430 holA 3530856..3531897 (-) 1042 Protein_3374 DNA polymerase III subunit delta -
  FGE23_RS17435 - 3531937..3532091 (-) 155 Protein_3375 hypothetical protein -
  FGE23_RS17440 - 3532132..3532266 (+) 135 WP_003152870.1 YqzM family protein -
  FGE23_RS17445 comEC 3532285..3534636 (-) 2352 WP_146277191.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  FGE23_RS17450 - 3534637..3535206 (-) 570 WP_003152868.1 ComE operon protein 2 -
  FGE23_RS17455 comEA 3535273..3535887 (-) 615 WP_146277193.1 helix-hairpin-helix domain-containing protein Machinery gene
  FGE23_RS17460 comER 3535946..3536767 (+) 822 WP_146277195.1 late competence protein ComER -
  FGE23_RS17465 - 3536836..3537573 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  FGE23_RS17470 rsfS 3537570..3537926 (-) 357 WP_003152864.1 ribosome silencing factor -
  FGE23_RS17475 yqeK 3537944..3538504 (-) 561 WP_146277197.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  FGE23_RS17480 - 3538494..3539063 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  FGE23_RS17485 yhbY 3539074..3539364 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  FGE23_RS17490 aroE 3539358..3540200 (-) 843 WP_015417858.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 6.0523

>NTDB_id=366394 FGE23_RS17455 WP_146277193.1 3535273..3535887(-) (comEA) [Bacillus amyloliquefaciens strain X030]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLRDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=366394 FGE23_RS17455 WP_146277193.1 3535273..3535887(-) (comEA) [Bacillus amyloliquefaciens strain X030]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCGGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.333

94.118

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment