Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   FED53_RS04745 Genome accession   NZ_CP040367
Coordinates   799616..799927 (+) Length   103 a.a.
NCBI ID   WP_025911556.1    Uniprot ID   -
Organism   Priestia flexa isolate 1-2-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 794616..804927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FED53_RS04715 (FED53_04715) - 795341..795514 (-) 174 WP_025911564.1 DUF2759 domain-containing protein -
  FED53_RS04720 (FED53_04720) - 795711..796343 (+) 633 WP_025911563.1 MBL fold metallo-hydrolase -
  FED53_RS04725 (FED53_04725) - 796386..796625 (-) 240 WP_025911561.1 DUF2626 domain-containing protein -
  FED53_RS04730 (FED53_04730) - 796905..797288 (+) 384 WP_061784685.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FED53_RS04735 (FED53_04735) comGA 797505..798563 (+) 1059 WP_025911559.1 competence type IV pilus ATPase ComGA Machinery gene
  FED53_RS04740 (FED53_04740) comGB 798544..799602 (+) 1059 WP_138116633.1 competence type IV pilus assembly protein ComGB -
  FED53_RS04745 (FED53_04745) comGC 799616..799927 (+) 312 WP_025911556.1 competence type IV pilus major pilin ComGC Machinery gene
  FED53_RS04750 (FED53_04750) comGD 799929..800363 (+) 435 WP_062686625.1 competence type IV pilus minor pilin ComGD -
  FED53_RS04755 (FED53_04755) - 800347..800682 (+) 336 WP_025911553.1 type II secretion system protein -
  FED53_RS04760 (FED53_04760) comGF 800679..801107 (+) 429 WP_025911552.1 competence type IV pilus minor pilin ComGF -
  FED53_RS04765 (FED53_04765) comGG 801070..801489 (+) 420 WP_061784683.1 competence type IV pilus minor pilin ComGG -
  FED53_RS04770 (FED53_04770) - 801568..802167 (-) 600 Protein_781 cupin domain-containing protein -
  FED53_RS04775 (FED53_04775) - 802312..802503 (+) 192 WP_025911548.1 YqzE family protein -
  FED53_RS04780 (FED53_04780) - 802541..803362 (-) 822 WP_025911547.1 YqhG family protein -

Sequence


Protein


Download         Length: 103 a.a.        Molecular weight: 11017.88 Da        Isoelectric Point: 5.7969

>NTDB_id=363688 FED53_RS04745 WP_025911556.1 799616..799927(+) (comGC) [Priestia flexa isolate 1-2-1]
MLKNEKGFTLIEMLIVMLVITVLLLIMVPNVLKHNAVINNKGCSALVKTVEAQVQVYELENGTKPTLNELESGGYLKEGT
VCPNGSKITIGTDGRVSASDGNS

Nucleotide


Download         Length: 312 bp        

>NTDB_id=363688 FED53_RS04745 WP_025911556.1 799616..799927(+) (comGC) [Priestia flexa isolate 1-2-1]
ATGTTAAAAAATGAGAAAGGTTTTACTTTAATTGAGATGCTCATTGTTATGTTAGTTATTACAGTGCTATTGCTTATTAT
GGTGCCAAATGTATTAAAGCATAACGCTGTTATTAACAATAAAGGATGTTCGGCTTTAGTGAAAACAGTGGAAGCACAAG
TGCAGGTGTATGAGCTTGAAAACGGAACAAAGCCTACTTTAAACGAGCTTGAAAGCGGAGGATACTTAAAAGAAGGAACG
GTGTGTCCAAATGGTTCGAAAATTACAATAGGTACGGATGGACGCGTATCAGCGAGTGATGGAAACTCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

53.684

92.233

0.495

  comGC Lactococcus lactis subsp. cremoris KW2

47.5

77.67

0.369

  comGC Staphylococcus aureus MW2

40.86

90.291

0.369

  comGC Staphylococcus aureus N315

40.86

90.291

0.369


Multiple sequence alignment