Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FD725_RS25240 Genome accession   NZ_CP040297
Coordinates   5858400..5860412 (+) Length   670 a.a.
NCBI ID   WP_179050683.1    Uniprot ID   -
Organism   Nostoc sp. TCL26-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5853400..5865412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD725_RS25225 (FD725_25195) dnaJ 5853541..5854668 (-) 1128 WP_179050680.1 molecular chaperone DnaJ -
  FD725_RS25230 (FD725_25200) dnaK 5855151..5857115 (-) 1965 WP_179050681.1 molecular chaperone DnaK -
  FD725_RS25235 (FD725_25205) grpE 5857311..5858048 (-) 738 WP_179050682.1 nucleotide exchange factor GrpE -
  FD725_RS25240 (FD725_25210) pilB/pilB1 5858400..5860412 (+) 2013 WP_179050683.1 GspE/PulE family protein Machinery gene
  FD725_RS25245 (FD725_25215) pilT 5860503..5861621 (+) 1119 WP_179050684.1 type IV pilus twitching motility protein PilT Machinery gene
  FD725_RS25250 (FD725_25220) - 5861701..5862924 (+) 1224 WP_179050685.1 type II secretion system F family protein -
  FD725_RS25255 (FD725_25225) - 5863022..5863552 (+) 531 WP_179050686.1 hypothetical protein -
  FD725_RS25260 (FD725_25230) - 5863573..5864880 (-) 1308 WP_179050687.1 hemolysin family protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74758.54 Da        Isoelectric Point: 5.3164

>NTDB_id=363039 FD725_RS25240 WP_179050683.1 5858400..5860412(+) (pilB/pilB1) [Nostoc sp. TCL26-01]
MTNSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTDQMRQALIESRKSGRPLTEVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVEFLDPEVNDVGSTMVGNLIDTLIPVDICRRHRLVPLSKNDEQTPPCVLVAMVDPDNLEASDDLNRILRPQGLTLN
RMVITQEDYQQLINHYLDELAVRQKHLEQEKFTDINQDLENLGNLDIQDAPDDMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKRIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPDTLAIVQDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCGDCRIAYTPTTEELARYGMTAAQEVAVTFYKANTVPAEAIAEAKAKNQLCPSCNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRHGATTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCHAQLQPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=363039 FD725_RS25240 WP_179050683.1 5858400..5860412(+) (pilB/pilB1) [Nostoc sp. TCL26-01]
ATGACTAACTCGTCACCACAACGACGTAGTACTGCTCTAACTACAAGAACAGAGTTTTCTCCCTTCGGGAATAAGCTAGT
ACAATCTGGCTACGTTAACACCGATCAGATGAGACAGGCGCTGATTGAGAGTCGCAAGTCTGGCAGACCCTTGACAGAAG
TGCTGGAGTCAATCACCGGGCGACAACTCTCACCTGAGTTCCTCAGACAGTACAAGAAACAGCAATTATTTGAATTAAAG
ATACTATACGGCGTTGAATTCCTTGATCCGGAAGTCAATGATGTCGGTAGCACGATGGTGGGTAATCTCATTGATACCCT
CATTCCGGTGGATATCTGTCGTCGTCATCGTTTAGTACCACTATCAAAAAATGACGAACAAACTCCGCCTTGTGTGTTAG
TAGCGATGGTCGATCCGGACAACTTAGAAGCGTCCGATGACCTGAATCGCATCCTCCGCCCACAAGGTTTGACATTAAAT
CGCATGGTAATTACCCAGGAGGATTACCAACAGCTAATCAATCACTATTTGGATGAATTAGCTGTCAGGCAAAAGCACCT
GGAACAAGAAAAATTTACCGATATTAATCAGGATTTAGAAAACCTCGGCAATCTGGATATTCAAGATGCGCCTGATGATA
TGGAGGCTGATTTAGGCGCAGCGATGAAGGGTGCGGAGGATGCACCAGTTATCAATCTTGTCAATAGAATTTTGGCTAAG
GCTTTGCATGAGAAAGTTTCTGATATCCATATTGAACCGCAAGAAGAAAATTTGCGTATCCGCTTCCGCAAGGATGGGGT
GTTGCGAGAAGCTTTTGATCCTCTACCAAAGAGAATCATTCCGGCGGTGACAGCCAGATTTAAAATTATCTCTAACTTAG
ATATTGCCGAGAGACGTTTACCCCAAGACGGACGGATTCGGCGGATGTTTGAGGGACGGAAAGTTGACTTCCGGGTGAAC
ACATTACCTAGTCGCTACGGTGAAAAGGTGGTATTGCGGATTTTGGATAACTCTTCTACCCAATTGGGTTTGAATAAGTT
AATCACCGATCCAGATACCTTGGCAATTGTTCAAGATATGGTGAGTAAGCCTTTTGGGTTGATTTTGGTAACAGGCCCTA
CTGGTTCTGGTAAAACAACTTCACTATATTCGGCACTGGCAGAGAAGAACTCACCGGGAATTAATATTAGTACTGTAGAA
GACCCCATCGAGTACAGTTTGCCGGGGATTACTCAAGTACAGGTAATTCGAGAAAAGGGTTTGGATTTTGCGACAGCCTT
ACGGGCTTTCTTGCGGCAAGATCCAGATGTGCTGCTGGTGGGGGAAACACGAGATAAGGAAACAGCAAAAACAGCGATTG
AAGCTGCTTTGACTGGTCACTTAGTATTAACTACTTTACATACTAATGATGCTCCAGGTGCGATCGCTCGTTTGGGTGAA
ATGGGAATTGAACCGTTCATGGTTTCTAGTTCCCTAATTGGTGTATTGGCACAACGTCTAGTCCGGCGTGTTTGTGGTGA
TTGTCGCATTGCTTATACTCCCACCACTGAAGAACTAGCGCGTTATGGGATGACAGCTGCTCAAGAGGTTGCCGTCACAT
TCTACAAGGCTAATACTGTACCAGCAGAAGCGATCGCTGAAGCTAAAGCCAAAAATCAGCTTTGCCCAAGTTGTAACGGT
GTTGGCTACAAAGGACGTTGTGGTGTCTATGAAGTGATGCGGGTGACAGAAAATCTGCAAACTTTAATCAACGAAGAAGC
ACCAACCGAACGCATCAAAGAAGTTGCTGTAGAAGAAGGCATGAAAACCTTGCTGGCTTACAGCTTAGATTTAGTCCGTC
ATGGTGCTACCACCCTAGAAGAAGTAGAACGGGTAACTTTCACCGACACAGGACTAGAAGCCGAATTAAAAGCTAAACGC
AAGAGTGGCCTTACCTGTCGGACTTGTCATGCCCAATTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACATCCAG
GTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.881

100

0.651
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.103

95.97

0.366

Multiple sequence alignment