Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   FD735_RS09635 Genome accession   NZ_CP040231
Coordinates   1866021..1867319 (-) Length   432 a.a.
NCBI ID   WP_139659069.1    Uniprot ID   A0A5B7Y957
Organism   Streptococcus sp. 1643     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1828278..1883933 1866021..1867319 within 0


Gene organization within MGE regions


Location: 1828278..1883933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD735_RS09435 (FD735_09435) pnuC 1828278..1829057 (-) 780 WP_139659051.1 nicotinamide riboside transporter PnuC -
  FD735_RS09440 (FD735_09440) - 1829067..1830125 (-) 1059 WP_139659052.1 AAA family ATPase -
  FD735_RS09445 (FD735_09445) clpC 1830271..1832703 (-) 2433 WP_139659053.1 ATP-dependent Clp protease ATP-binding subunit Regulator
  FD735_RS09450 (FD735_09450) - 1832705..1833163 (-) 459 WP_001211258.1 CtsR family transcriptional regulator -
  FD735_RS09455 (FD735_09455) - 1833492..1833812 (-) 321 WP_125391513.1 hypothetical protein -
  FD735_RS09460 (FD735_09460) - 1833802..1834263 (-) 462 WP_125391512.1 Panacea domain-containing protein -
  FD735_RS09975 - 1834405..1834755 (-) 351 WP_255296286.1 hypothetical protein -
  FD735_RS09470 (FD735_09470) - 1834771..1835061 (-) 291 WP_139659054.1 hypothetical protein -
  FD735_RS09475 (FD735_09475) - 1835111..1835323 (-) 213 WP_033630508.1 hypothetical protein -
  FD735_RS09480 (FD735_09480) - 1835592..1835933 (-) 342 WP_139659119.1 HNH endonuclease -
  FD735_RS09485 (FD735_09485) - 1836115..1836762 (-) 648 WP_139659055.1 hypothetical protein -
  FD735_RS09490 (FD735_09490) - 1837282..1838736 (-) 1455 WP_125391509.1 virulence-associated E family protein -
  FD735_RS09495 (FD735_09495) - 1838729..1839244 (-) 516 WP_125391508.1 hypothetical protein -
  FD735_RS09500 (FD735_09500) - 1839241..1839504 (-) 264 WP_125391507.1 hypothetical protein -
  FD735_RS09980 (FD735_09505) - 1839506..1839715 (-) 210 WP_125391506.1 hypothetical protein -
  FD735_RS09985 (FD735_09510) - 1839717..1839920 (-) 204 WP_084930585.1 hypothetical protein -
  FD735_RS09835 - 1840129..1840278 (-) 150 WP_162837410.1 hypothetical protein -
  FD735_RS09515 (FD735_09515) - 1840281..1840709 (-) 429 WP_139659056.1 hypothetical protein -
  FD735_RS09520 (FD735_09520) - 1840829..1841083 (-) 255 WP_125391504.1 hypothetical protein -
  FD735_RS09525 (FD735_09525) - 1841338..1841523 (-) 186 WP_000909283.1 hypothetical protein -
  FD735_RS09530 (FD735_09530) - 1841679..1842263 (+) 585 WP_139659057.1 helix-turn-helix transcriptional regulator -
  FD735_RS09535 (FD735_09535) - 1842298..1842567 (+) 270 WP_004261908.1 DUF4177 domain-containing protein -
  FD735_RS09540 (FD735_09540) - 1842828..1843292 (+) 465 WP_049510243.1 hypothetical protein -
  FD735_RS09545 (FD735_09545) - 1843285..1844073 (+) 789 WP_049510244.1 DUF1828 domain-containing protein -
  FD735_RS09555 (FD735_09555) - 1844479..1845645 (+) 1167 WP_139659058.1 site-specific integrase -
  FD735_RS09560 (FD735_09560) - 1845965..1848832 (-) 2868 WP_139659059.1 Spy0128 family protein -
  FD735_RS09565 (FD735_09565) - 1849038..1849766 (-) 729 WP_139659060.1 ABC transporter ATP-binding protein -
  FD735_RS09570 (FD735_09570) - 1849766..1850773 (-) 1008 WP_139659061.1 ABC transporter substrate-binding protein -
  FD735_RS09575 (FD735_09575) - 1850811..1851569 (-) 759 WP_139659062.1 ABC transporter permease -
  FD735_RS09580 (FD735_09580) - 1851532..1851822 (-) 291 WP_139659063.1 thiamine-binding protein -
  FD735_RS09585 (FD735_09585) polA 1852101..1854734 (-) 2634 WP_139659064.1 DNA polymerase I -
  FD735_RS09590 (FD735_09590) - 1854865..1857894 (-) 3030 WP_139659065.1 SIALI-17 repeat-containing surface protein -
  FD735_RS09595 (FD735_09595) - 1858010..1859131 (-) 1122 WP_139659066.1 CHAP domain-containing protein -
  FD735_RS09600 (FD735_09600) - 1859231..1859497 (-) 267 WP_001278165.1 Veg family protein -
  FD735_RS09605 (FD735_09605) dnaB 1859499..1860851 (-) 1353 WP_000852476.1 replicative DNA helicase -
  FD735_RS09610 (FD735_09610) rplI 1860895..1861347 (-) 453 WP_000864208.1 50S ribosomal protein L9 -
  FD735_RS09615 (FD735_09615) - 1861344..1863317 (-) 1974 WP_139659067.1 DHH family phosphoesterase -
  FD735_RS09620 (FD735_09620) - 1863474..1864652 (-) 1179 WP_139659120.1 acetyl-CoA C-acetyltransferase -
  FD735_RS09625 (FD735_09625) raiA 1864734..1865282 (-) 549 WP_000599111.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FD735_RS09630 (FD735_09630) comFC/cflB 1865362..1866024 (-) 663 WP_139659068.1 ComF family protein Machinery gene
  FD735_RS09635 (FD735_09635) comFA/cflA 1866021..1867319 (-) 1299 WP_139659069.1 DEAD/DEAH box helicase Machinery gene
  FD735_RS09640 (FD735_09640) - 1867376..1868011 (+) 636 WP_139659070.1 YigZ family protein -
  FD735_RS09645 (FD735_09645) - 1868026..1868469 (+) 444 WP_139659071.1 PH domain-containing protein -
  FD735_RS09650 (FD735_09650) cysK 1868566..1869492 (+) 927 WP_139659072.1 cysteine synthase A -
  FD735_RS09655 (FD735_09655) tsf 1869596..1870636 (-) 1041 WP_139659073.1 translation elongation factor Ts -
  FD735_RS09660 (FD735_09660) rpsB 1870715..1871494 (-) 780 WP_000268475.1 30S ribosomal protein S2 -
  FD735_RS09665 (FD735_09665) pcsB 1871717..1872928 (-) 1212 WP_139659074.1 peptidoglycan hydrolase PcsB -
  FD735_RS09670 (FD735_09670) mreD 1873022..1873516 (-) 495 WP_049477904.1 rod shape-determining protein MreD -
  FD735_RS09675 (FD735_09675) mreC 1873519..1874334 (-) 816 WP_139659075.1 rod shape-determining protein MreC -
  FD735_RS09680 (FD735_09680) - 1874395..1875189 (-) 795 WP_139659076.1 energy-coupling factor transporter transmembrane protein EcfT -
  FD735_RS09685 (FD735_09685) - 1875182..1876021 (-) 840 WP_084944240.1 energy-coupling factor transporter ATPase -
  FD735_RS09690 (FD735_09690) - 1876006..1876833 (-) 828 WP_139659077.1 energy-coupling factor ABC transporter ATP-binding protein -
  FD735_RS09695 (FD735_09695) pgsA 1876830..1877375 (-) 546 WP_139659078.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  FD735_RS09700 (FD735_09700) rodZ 1877386..1878207 (-) 822 WP_176553070.1 cytoskeleton protein RodZ -
  FD735_RS09705 (FD735_09705) - 1878243..1879526 (-) 1284 WP_125391774.1 EF-P 5-aminopentanol modification-associated protein YfmH -
  FD735_RS09710 (FD735_09710) - 1879523..1880773 (-) 1251 WP_139659080.1 EF-P 5-aminopentanol modification-associated protein YfmF -
  FD735_RS09715 (FD735_09715) yaaA 1880934..1881302 (+) 369 WP_139659081.1 S4 domain-containing protein YaaA -
  FD735_RS09720 (FD735_09720) recF 1881305..1882396 (+) 1092 WP_139659082.1 DNA replication/repair protein RecF -
  FD735_RS09725 (FD735_09725) guaB 1882455..1883933 (-) 1479 WP_071851777.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49709.71 Da        Isoelectric Point: 8.3637

>NTDB_id=362677 FD735_RS09635 WP_139659069.1 1866021..1867319(-) (comFA/cflA) [Streptococcus sp. 1643]
MKVNPNYLGRLFTEKELTEEERQEAVKLPAMRKEKGKLFCQRCNSLILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
ENFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQEPILVHAVTGAGKTEMIYQVVAKVIDEGGAVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFYHAFDLLIVDEVDAFPYVDNSVLYYAVNQCVKEEGLRIFLTATST
DELDKKVRTGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYIEKSQLSPKLKSYIKKQRRTSYPLLIFASEIKKGEKLKEL
LQEQFPNENIGFVSSITENRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLFFHDGLNVSIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=362677 FD735_RS09635 WP_139659069.1 1866021..1867319(-) (comFA/cflA) [Streptococcus sp. 1643]
ATGAAAGTAAATCCAAATTACCTCGGTCGCTTGTTTACTGAGAAAGAATTAACTGAAGAAGAACGACAGGAAGCAGTGAA
ACTGCCAGCAATGAGAAAAGAGAAGGGGAAACTGTTTTGTCAACGTTGTAATAGTTTGATTCTAGAAGAATGGTATTTGC
CTATTGGCGCCTACTATTGCAGGGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCGCAG
GAGAATTTTCCTAAGCAAGATGTTCTCAAATGGCGTGGTCAGTTAACCCCTTTTCAAGAAAAAGTGTCAGAGGGACTGCT
TCAAGCGGTGGACAAGCAAGAGCCAATCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAGATGATTTACCAGGTTG
TGGCTAAGGTGATTGATGAAGGTGGTGCAGTTTGTTTAGCCAGCCCTCGAATTGATGTGTGTTTGGAGCTGTATAAACGA
CTGCAGAATGACTTTGCTTGTGAGATAGCACTACTTCATGGTGAGTCAGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACGACTCATCAGCTGTTAAAATTTTATCATGCTTTTGACTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAACTCTGTTCTTTACTATGCTGTAAACCAATGTGTAAAGGAGGAGGGGCTAAGGATATTTCTTACAGCGACCTCTACA
GATGAGTTAGATAAGAAGGTTCGCACTGGAGAATTAAAACGATTGAGCTTGCCAAGACGATTTCATGGAAATCCATTGAT
TATTCCAAAACCAGTTTGGTTATCAGACTTTAATCGCTATATAGAAAAGAGTCAGTTGTCTCCAAAGTTAAAGTCCTACA
TTAAGAAGCAGAGAAGAACAAGTTATCCGTTGTTAATCTTTGCATCTGAGATTAAGAAAGGCGAGAAACTGAAAGAACTC
TTGCAGGAACAGTTTCCAAATGAAAACATCGGATTTGTGTCCTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAGTTGACAATCCTTATTAGTACGACAATTTTGGAGCGTGGGGTCACCTTCCCTTGTGTGGATGTTTTCG
TTGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGCTTGATTCAGATTGGAGGGCGAGTTGGGCGCAGTATGGATAGA
CCGACTGGTGAATTGCTCTTCTTTCATGATGGATTAAATGTTTCCATTAAAAAAGCAATCAAGGAAATAAAGCAGATGAA
CAAGGAGGCAGGCTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7Y957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

91.204

100

0.912
  comFA/cflA Streptococcus pneumoniae Rx1

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae D39

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae R6

89.815

100

0.898
  comFA/cflA Streptococcus mitis SK321

89.583

100

0.896
  comFA/cflA Streptococcus pneumoniae TIGR4

89.583

100

0.896
  comFA Lactococcus lactis subsp. cremoris KW2

50.732

94.907

0.481
  comFA Latilactobacillus sakei subsp. sakei 23K

38.864

100

0.396

Multiple sequence alignment