Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   FCJ76_RS12860 Genome accession   NZ_CP039935
Coordinates   2489733..2490350 (-) Length   205 a.a.
NCBI ID   WP_137200853.1    Uniprot ID   -
Organism   Bacillus subtilis strain H19     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2484733..2495350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCJ76_RS12830 rpsT 2485035..2485301 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  FCJ76_RS12835 holA 2485316..2486359 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  FCJ76_RS12840 - 2486399..2486548 (-) 150 WP_120363468.1 hypothetical protein -
  FCJ76_RS12845 yqzM 2486589..2486723 (+) 135 WP_003229983.1 YqzM family protein -
  FCJ76_RS12850 comEC 2486763..2489093 (-) 2331 WP_137200851.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  FCJ76_RS12855 comEB 2489097..2489666 (-) 570 WP_003229978.1 ComE operon protein 2 -
  FCJ76_RS12860 comEA 2489733..2490350 (-) 618 WP_137200853.1 competence protein ComEA Machinery gene
  FCJ76_RS12865 comER 2490434..2491255 (+) 822 WP_134981848.1 late competence protein ComER -
  FCJ76_RS12870 yqeM 2491321..2492064 (-) 744 WP_033884459.1 class I SAM-dependent methyltransferase -
  FCJ76_RS12875 rsfS 2492061..2492417 (-) 357 WP_014480315.1 ribosome silencing factor -
  FCJ76_RS12880 yqeK 2492435..2492995 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  FCJ76_RS12885 nadD 2492985..2493554 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  FCJ76_RS12890 yhbY 2493566..2493856 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  FCJ76_RS12895 aroE 2493850..2494692 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21786.48 Da        Isoelectric Point: 4.7284

>NTDB_id=361144 FCJ76_RS12860 WP_137200853.1 2489733..2490350(-) (comEA) [Bacillus subtilis strain H19]
MNWLNQHKKTIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=361144 FCJ76_RS12860 WP_137200853.1 2489733..2490350(-) (comEA) [Bacillus subtilis strain H19]
ATGAATTGGTTGAATCAGCATAAGAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment