Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GUK18_RS10885 Genome accession   NZ_CP047600
Coordinates   2253746..2254405 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O84:H7 strain Trh52     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2248746..2259405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS10870 (GUK18_10900) ygiS 2250951..2252558 (-) 1608 WP_001305104.1 ABC transporter substrate-binding protein -
  GUK18_RS10875 (GUK18_10905) ygiV 2252667..2253149 (-) 483 WP_000183493.1 GyrI-like domain-containing protein -
  GUK18_RS10880 (GUK18_10910) ygiW 2253202..2253594 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GUK18_RS10885 (GUK18_10915) ciaR 2253746..2254405 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GUK18_RS10890 (GUK18_10920) qseC 2254402..2255751 (+) 1350 WP_000673388.1 quorum sensing histidine kinase QseC -
  GUK18_RS10895 (GUK18_10925) mdaB 2255861..2256442 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GUK18_RS10900 (GUK18_10930) ygiN 2256473..2256787 (+) 315 WP_000633738.1 putative quinol monooxygenase -
  GUK18_RS10905 (GUK18_10935) - 2256832..2257719 (-) 888 WP_000614943.1 MurR/RpiR family transcriptional regulator -
  GUK18_RS10910 (GUK18_10940) - 2257716..2258663 (-) 948 WP_001296418.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=360335 GUK18_RS10885 WP_001221493.1 2253746..2254405(+) (ciaR) [Escherichia coli O84:H7 strain Trh52]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=360335 GUK18_RS10885 WP_001221493.1 2253746..2254405(+) (ciaR) [Escherichia coli O84:H7 strain Trh52]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365