Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FAZ90_RS01250 Genome accession   NZ_CP039700
Coordinates   266732..267757 (+) Length   341 a.a.
NCBI ID   WP_016767986.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain ECSMB14105     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261732..272757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAZ90_RS01230 (FAZ90_01230) - 262083..262811 (-) 729 WP_010435300.1 glutathione peroxidase -
  FAZ90_RS01240 (FAZ90_01240) oxyR 263033..263926 (+) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  FAZ90_RS01245 (FAZ90_01245) - 264022..266580 (-) 2559 WP_032547293.1 penicillin-binding protein 1A -
  FAZ90_RS01250 (FAZ90_01250) pilM 266732..267757 (+) 1026 WP_016767986.1 type IV pilus assembly protein PilM Machinery gene
  FAZ90_RS01255 (FAZ90_01255) pilN 267741..268355 (+) 615 WP_046209435.1 PilN domain-containing protein Machinery gene
  FAZ90_RS01260 (FAZ90_01260) pilO 268348..268977 (+) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  FAZ90_RS01265 (FAZ90_01265) pilP 268964..269482 (+) 519 WP_016767989.1 pilus assembly protein PilP Machinery gene
  FAZ90_RS01270 (FAZ90_01270) pilQ 269542..271233 (+) 1692 WP_046209434.1 type IV pilus secretin PilQ Machinery gene
  FAZ90_RS01275 (FAZ90_01275) aroK 271410..271928 (+) 519 WP_009848907.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38112.77 Da        Isoelectric Point: 6.8348

>NTDB_id=360160 FAZ90_RS01250 WP_016767986.1 266732..267757(+) (pilM) [Vibrio cyclitrophicus strain ECSMB14105]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGATSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=360160 FAZ90_RS01250 WP_016767986.1 266732..267757(+) (pilM) [Vibrio cyclitrophicus strain ECSMB14105]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGAGCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.097

99.413

0.528
  pilM Vibrio campbellii strain DS40M4

47.661

100

0.478

Multiple sequence alignment