Detailed information NTG0035983

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: I33_RS08860
• Genome accession: NC_017195
• Coordinates: 1750222..1752798 (+)
• Length: 858 a.a.
• NCBI ID: WP_014476844.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis str. RO-NN-1
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1745222..1757798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I33_RS08840 (I33_1886)	kbl	1745994..1747172 (+)	1179	WP_014476842.1	glycine C-acetyltransferase	-
I33_RS08845	miaB	1747320..1748849 (+)	1530	WP_014479820.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
I33_RS08850 (I33_1888)	ymcA	1748851..1749282 (+)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
I33_RS08855 (I33_1889)	cotE	1749544..1750089 (+)	546	WP_003231833.1	outer spore coat protein CotE	-
I33_RS08860 (I33_1890)	hexA	1750222..1752798 (+)	2577	WP_014476844.1	DNA mismatch repair protein MutS	Machinery gene
I33_RS08865 (I33_1891)	hexB	1752814..1754697 (+)	1884	WP_014476845.1	DNA mismatch repair endonuclease MutL	Machinery gene
I33_RS08870 (I33_1892)	ymzD	1755095..1755550 (-)	456	WP_014476846.1	regulatory YrvL family protein	-
I33_RS08875 (I33_1893)	-	1755685..1756242 (-)	558	WP_014476847.1	hypothetical protein	-
I33_RS08880 (I33_1894)	pksA	1756363..1756980 (+)	618	WP_014476848.1	TetR/AcrR family transcriptional regulator	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97692.84 Da
• Isoelectric Point: 5.2035

>NTDB_id=35983 I33_RS08860 WP_014476844.1 1750222..1752798(+) (hexA) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFMRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAERIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQLLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNIHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPEDLIARAQDILKELEHSGNKPEVPVQKPQV
KEKPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=35983 I33_RS08860 WP_014476844.1 1750222..1752798(+) (hexA) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTATGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGACG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGATACAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTAGTTCAGCT
GATTACGCCCGGAACTGTAATGGACGGCAAAGGCATCCATGAGTCGGAAAATAATTTTATCGCATCTGTTTCAGCCTGCT
CGAACGGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACGATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTATGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTGTACTCAAAGCGTAATCTGGAGCTGACCGAAACGATCCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATCAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTAATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTGTATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTCGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTTCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAGAGCGCATTGACCCTTGCGGGGATGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGCCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGTTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAATTTGCATTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCAGAGCGCTACATTACCCCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCAGAAAATAA
CATCTGCGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTGCAGTTGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGCCACTACACGAAACCGGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACGGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCGAAAAAAGCGGTGCTCCCGATTTTCGATCAA
ATTTTTACGCGAATCGGCGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAATGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCTCAGCTGAAAAACATTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATCAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGAAGATCTGA
TTGCGCGCGCTCAAGATATTTTAAAAGAGCTTGAGCATTCAGGAAACAAACCGGAAGTGCCGGTGCAGAAACCTCAGGTG
AAAGAAAAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATTGATGCCTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

• MutS domain V (MutS_V) (598-785)

• MutS domain I (MutS_I) (5-116)

• MutS domain II (MutS_II) (125-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.357	99.65	0.492
mutS	Pseudomonas stutzeri strain ATCC 17587	38.908	100	0.399