Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   GSY53_RS16350 Genome accession   NZ_CP047325
Coordinates   3157429..3159477 (+) Length   682 a.a.
NCBI ID   WP_003244692.1    Uniprot ID   O34942
Organism   Bacillus subtilis strain GOT9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3152429..3164477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSY53_RS16320 (GSY53_16320) spoVM 3153065..3153145 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  GSY53_RS16325 (GSY53_16325) rpmB 3153218..3153406 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  GSY53_RS16330 (GSY53_16330) yloU 3153683..3154045 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  GSY53_RS16335 (GSY53_16335) fakA 3154061..3155722 (+) 1662 WP_003232052.1 DAK2 domain-containing protein -
  GSY53_RS16340 (GSY53_16340) sdaAB 3155861..3156523 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  GSY53_RS16345 (GSY53_16345) sdaAA 3156549..3157451 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  GSY53_RS16350 (GSY53_16350) recG 3157429..3159477 (+) 2049 WP_003244692.1 ATP-dependent DNA helicase RecG Machinery gene
  GSY53_RS16355 (GSY53_16355) fapR 3159586..3160152 (+) 567 WP_003232044.1 transcription factor FapR -
  GSY53_RS16360 (GSY53_16360) plsX 3160166..3161167 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  GSY53_RS16365 (GSY53_16365) fabD 3161186..3162139 (+) 954 WP_003245314.1 ACP S-malonyltransferase -
  GSY53_RS16370 (GSY53_16370) fabG 3162132..3162872 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  GSY53_RS16375 (GSY53_16375) acpP 3162956..3163189 (+) 234 WP_003154310.1 acyl carrier protein -
  GSY53_RS16380 (GSY53_16380) rncS 3163329..3164078 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78140.72 Da        Isoelectric Point: 7.4260

>NTDB_id=359371 GSY53_RS16350 WP_003244692.1 3157429..3159477(+) (recG) [Bacillus subtilis strain GOT9]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=359371 GSY53_RS16350 WP_003244692.1 3157429..3159477(+) (recG) [Bacillus subtilis strain GOT9]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34942

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

100

100

1
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38