Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E5Q53_RS03845 Genome accession   NZ_CP038817
Coordinates   827831..828370 (+) Length   179 a.a.
NCBI ID   WP_005707111.1    Uniprot ID   -
Organism   Haemophilus parahaemolyticus strain NEB129     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 822831..833370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q53_RS03830 (E5Q53_03825) pssA 824117..825475 (+) 1359 WP_005707142.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  E5Q53_RS03835 (E5Q53_03830) rlmB 825563..826303 (+) 741 WP_005707158.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E5Q53_RS03840 (E5Q53_03835) - 826616..827776 (+) 1161 WP_005707123.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  E5Q53_RS03845 (E5Q53_03840) pilF 827831..828370 (+) 540 WP_005707111.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E5Q53_RS03850 (E5Q53_03845) - 828386..830713 (+) 2328 WP_005707139.1 Tex family protein -
  E5Q53_RS03865 (E5Q53_03860) - 831211..831864 (+) 654 WP_005707124.1 VWA domain-containing protein -
  E5Q53_RS03870 (E5Q53_03865) - 831864..832814 (+) 951 WP_005707146.1 kinase -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20479.20 Da        Isoelectric Point: 8.0949

>NTDB_id=355766 E5Q53_RS03845 WP_005707111.1 827831..828370(+) (pilF) [Haemophilus parahaemolyticus strain NEB129]
MKFVIFSKILTACAVLFLAGCVSSQIDPEFNRSEALKARINLALAYLEQQDYPKAKENIDKAIAHDRNDYLPYSVLAYYY
QQTGDVANSQNAYQKALKLSQNRPDVLNNYGTFLCKQGQFQQAYSQFEQALQSKHAYYHQADTLENIILCAKSEPNSQKF
NEAFAQLEKLDKIKADKLR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=355766 E5Q53_RS03845 WP_005707111.1 827831..828370(+) (pilF) [Haemophilus parahaemolyticus strain NEB129]
ATGAAATTCGTAATCTTTTCAAAAATTTTAACCGCTTGTGCTGTGCTATTTTTAGCAGGTTGCGTTTCCTCCCAAATCGA
TCCGGAATTTAATCGCTCGGAAGCCTTAAAAGCTCGAATCAATCTTGCCCTTGCCTATTTAGAACAACAGGATTACCCAA
AAGCCAAGGAAAATATCGATAAAGCGATTGCTCACGATAGAAACGATTACCTGCCTTATTCAGTGCTGGCGTATTATTAT
CAACAAACAGGAGATGTAGCCAACAGTCAGAACGCCTATCAAAAAGCATTGAAACTCAGCCAAAATCGCCCAGATGTGCT
CAATAATTACGGCACTTTTTTATGTAAGCAAGGTCAATTCCAACAAGCTTATTCACAGTTTGAACAAGCCCTCCAAAGCA
AGCACGCTTACTATCATCAAGCCGATACGCTTGAAAATATCATTCTCTGTGCAAAATCAGAGCCCAATTCGCAAAAATTT
AATGAAGCATTCGCCCAGTTGGAAAAATTAGATAAAATAAAGGCAGATAAGCTCCGTTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(92-123)

Tetratricopeptide repeat (TPR_8)

(37-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.366

100

0.648
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.349

96.089

0.436

Multiple sequence alignment