Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY25_RS16210 Genome accession   NZ_CP046779
Coordinates   3246562..3247014 (+) Length   150 a.a.
NCBI ID   WP_005493986.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2014V-1066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3241562..3252014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY25_RS16195 pdhR 3243594..3244361 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GPY25_RS16200 ampD 3244767..3245318 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY25_RS16205 nadC 3245411..3246298 (+) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY25_RS16210 pilA 3246562..3247014 (+) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY25_RS16215 pilB 3247016..3248701 (+) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY25_RS16220 pilC 3248725..3249948 (+) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY25_RS16225 pilD 3250013..3250882 (+) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY25_RS16230 coaE 3250883..3251497 (+) 615 WP_005493969.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.99 Da        Isoelectric Point: 7.1316

>NTDB_id=355499 GPY25_RS16210 WP_005493986.1 3246562..3247014(+) (pilA) [Vibrio parahaemolyticus strain 2014V-1066]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPDSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=355499 GPY25_RS16210 WP_005493986.1 3246562..3247014(+) (pilA) [Vibrio parahaemolyticus strain 2014V-1066]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTGATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373