Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SaO268_RS08215 Genome accession   NZ_CP038612
Coordinates   1616974..1617948 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain O268     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1611974..1622948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SaO268_RS08175 (SaO268_1507) gcvT 1612476..1613567 (-) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  SaO268_RS08180 (SaO268_1508) - 1613725..1614249 (-) 525 WP_001015120.1 shikimate kinase -
  SaO268_RS08185 - 1614239..1614385 (-) 147 WP_001789879.1 hypothetical protein -
  SaO268_RS08190 (SaO268_1509) comGF 1614482..1614979 (-) 498 WP_078061106.1 competence type IV pilus minor pilin ComGF Machinery gene
  SaO268_RS08195 (SaO268_1510) comGE 1614897..1615196 (-) 300 WP_000844409.1 hypothetical protein Machinery gene
  SaO268_RS08200 (SaO268_1511) comGD 1615183..1615629 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  SaO268_RS08205 (SaO268_1512) comGC 1615607..1615918 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SaO268_RS08210 (SaO268_1513) comGB 1615932..1617002 (-) 1071 WP_000776418.1 competence type IV pilus assembly protein ComGB Machinery gene
  SaO268_RS08215 (SaO268_1514) comGA 1616974..1617948 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SaO268_RS08220 (SaO268_1515) - 1618000..1618623 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SaO268_RS08225 (SaO268_1516) - 1618620..1618949 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SaO268_RS08230 (SaO268_1517) - 1618949..1619935 (-) 987 WP_135830986.1 ROK family glucokinase -
  SaO268_RS08235 - 1619932..1620135 (-) 204 WP_000087561.1 YqgQ family protein -
  SaO268_RS08240 (SaO268_1518) - 1620116..1621579 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  SaO268_RS08245 (SaO268_1519) - 1621591..1622130 (-) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  SaO268_RS08250 (SaO268_1520) rpmG 1622339..1622488 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=355189 SaO268_RS08215 WP_000697220.1 1616974..1617948(-) (comGA) [Staphylococcus aureus strain O268]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=355189 SaO268_RS08215 WP_000697220.1 1616974..1617948(-) (comGA) [Staphylococcus aureus strain O268]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment