Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GO004_RS00710 Genome accession   NZ_CP046591
Coordinates   111724..112341 (+) Length   205 a.a.
NCBI ID   WP_088467267.1    Uniprot ID   -
Organism   Bacillus subtilis strain R31     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 106724..117341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO004_RS00675 (GO004_00665) aroE 107382..108224 (+) 843 WP_088467268.1 shikimate dehydrogenase -
  GO004_RS00680 (GO004_00670) yhbY 108218..108508 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GO004_RS00685 (GO004_00675) nadD 108520..109089 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GO004_RS00690 (GO004_00680) yqeK 109079..109639 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GO004_RS00695 (GO004_00685) rsfS 109657..110013 (+) 357 WP_014477391.1 ribosome silencing factor -
  GO004_RS00700 (GO004_00690) yqeM 110010..110753 (+) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  GO004_RS00705 (GO004_00695) comER 110819..111640 (-) 822 WP_004398597.1 late competence protein ComER -
  GO004_RS00710 (GO004_00700) comEA 111724..112341 (+) 618 WP_088467267.1 competence protein ComEA Machinery gene
  GO004_RS00715 (GO004_00705) comEB 112408..112977 (+) 570 WP_003229978.1 ComE operon protein 2 -
  GO004_RS00720 (GO004_00710) comEC 112981..115311 (+) 2331 WP_032677118.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GO004_RS00725 (GO004_00715) yqzM 115351..115485 (-) 135 WP_003229983.1 YqzM family protein -
  GO004_RS00730 (GO004_00720) - 115526..115675 (+) 150 WP_003229985.1 hypothetical protein -
  GO004_RS00735 (GO004_00725) holA 115715..116758 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  GO004_RS00740 (GO004_00730) rpsT 116773..117039 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21840.58 Da        Isoelectric Point: 4.8354

>NTDB_id=353528 GO004_RS00710 WP_088467267.1 111724..112341(+) (comEA) [Bacillus subtilis strain R31]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVRHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=353528 GO004_RS00710 WP_088467267.1 111724..112341(+) (comEA) [Bacillus subtilis strain R31]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGTGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGGCCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

36.364

100

0.39