Detailed information NTG0352859

Bioinformatically predicted

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: E4K03_RS03810
• Genome accession: NZ_CP038262
• Coordinates: 781797..782537 (+)
• Length: 246 a.a.
• NCBI ID: WP_000076101.1
• Uniprot ID: N9LH62
• Organism: Acinetobacter baumannii strain LEV1449/17Ec
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	783490..784709	781797..782537	flank	953

Gene organization within MGE regions

Location: 781797..784709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4K03_RS03810 (E4K03_03810)	pilO	781797..782537 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
E4K03_RS03815 (E4K03_03815)	pilP	782548..783075 (+)	528	WP_004833663.1	pilus assembly protein PilP	Machinery gene
E4K03_RS03820 (E4K03_03820)	-	783138..783437 (+)	300	Protein_726	AMIN domain-containing protein	-
E4K03_RS03825 (E4K03_03825)	-	783490..784709 (+)	1220	WP_087486619.1	IS3-like element ISAba19 family transposase	-

Sequence

Protein

• Length: 246 a.a.
• Molecular weight: 27819.59 Da
• Isoelectric Point: 4.8357

>NTDB_id=352859 E4K03_RS03810 WP_000076101.1 781797..782537(+) (pilO) [Acinetobacter baumannii strain LEV1449/17Ec]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide

• Length: 741 bp

>NTDB_id=352859 E4K03_RS03810 WP_000076101.1 781797..782537(+) (pilO) [Acinetobacter baumannii strain LEV1449/17Ec]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (73-220)

3D structure

• AlphaFold DB: N9LH62

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilO	Acinetobacter baumannii D1279779	100	100	1
comO	Acinetobacter baylyi ADP1	69.421	98.374	0.683