Detailed information NTG0352771

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: E3983_RS06375
• Genome accession: NZ_CP038254
• Coordinates: 1407826..1408890 (+)
• Length: 354 a.a.
• NCBI ID: WP_058501754.1
• Uniprot ID: A0A0W0VUE9
• Organism: Legionella israelensis strain HL-0427-4011
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1402826..1413890

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3983_RS06365 (E3983_06365)	-	1404010..1406373 (-)	2364	WP_135060291.1	penicillin-binding protein 1A	-
E3983_RS06370 (E3983_06370)	-	1406564..1407568 (-)	1005	WP_135060292.1	hypothetical protein	-
E3983_RS06375 (E3983_06375)	pilM	1407826..1408890 (+)	1065	WP_058501754.1	type IV pilus assembly protein PilM	Machinery gene
E3983_RS06380 (E3983_06380)	pilN	1408894..1409466 (+)	573	WP_058501753.1	PilN domain-containing protein	Machinery gene
E3983_RS06385 (E3983_06385)	pilO	1409478..1410077 (+)	600	WP_058501752.1	type 4a pilus biogenesis protein PilO	Machinery gene
E3983_RS06390 (E3983_06390)	-	1410074..1410607 (+)	534	WP_135060293.1	pilus assembly protein PilP	-
E3983_RS06395 (E3983_06395)	pilQ	1410610..1412751 (+)	2142	WP_135060294.1	type IV pilus secretin PilQ family protein	Machinery gene
E3983_RS06400 (E3983_06400)	aroK	1412833..1413354 (+)	522	WP_172460999.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 39008.06 Da
• Isoelectric Point: 4.5440

>NTDB_id=352771 E3983_RS06375 WP_058501754.1 1407826..1408890(+) (pilM) [Legionella israelensis strain HL-0427-4011]
MLKLLKPKKKSILGIDVSSTSVKILEISGEAPNYQLNAYASELLPEKAVEANVIKDVDAVAGCIKRATSRAKFSTKLAAA
AVPDSAVINKVIQINEGLNDEEMEELVVMEADKYIPYPIDEINIDFEILGPSAKNSTMLDVLIVASRAENVSNRAEVLTQ
AGLEAKIVDVESYAVERAVQLIAPDLPAEGVDKVIAVIDVGANYTHLFVLHGMKTVFSREEEFGARLLIESLAQRYEMTY
EQALEAYMQNQLLEDYKETVLEPFQEMMLLQVKRTLQFFYSTSNHGFVDHILLAGGLARQEGLVDLIQQQLGVPTTIANP
FKHMQLGKMLNSEVLNKEAPALLVACGLALRSIE

Nucleotide

• Length: 1065 bp

>NTDB_id=352771 E3983_RS06375 WP_058501754.1 1407826..1408890(+) (pilM) [Legionella israelensis strain HL-0427-4011]
GTGCTCAAATTGCTTAAGCCTAAAAAGAAATCAATATTGGGTATAGACGTCAGCTCAACTTCCGTTAAGATCCTGGAGAT
TTCTGGTGAGGCTCCGAATTATCAGTTGAATGCTTATGCCAGCGAACTTTTGCCCGAAAAAGCGGTAGAAGCGAATGTAA
TTAAGGATGTTGATGCGGTAGCTGGATGCATTAAAAGAGCAACTTCTCGCGCAAAATTTTCCACTAAACTAGCGGCTGCA
GCGGTACCTGACTCCGCTGTCATTAATAAGGTAATACAAATCAATGAAGGCTTAAATGACGAGGAGATGGAAGAACTGGT
CGTCATGGAAGCTGATAAATATATCCCTTACCCTATAGATGAAATTAATATTGATTTTGAAATCCTTGGGCCTTCAGCAA
AAAATTCCACCATGCTTGATGTGTTGATTGTCGCATCCCGAGCGGAAAATGTAAGTAATCGAGCTGAGGTACTTACCCAG
GCAGGTCTTGAGGCTAAAATAGTTGATGTTGAATCTTATGCTGTCGAACGGGCAGTACAGTTAATAGCCCCTGATCTTCC
GGCGGAAGGGGTAGATAAAGTTATTGCTGTGATTGATGTAGGAGCCAACTACACGCATTTATTTGTTTTGCATGGAATGA
AAACGGTGTTTTCCCGTGAAGAGGAATTTGGTGCCCGGCTTCTCATTGAATCATTGGCACAGCGATATGAAATGACCTAC
GAACAGGCTCTGGAAGCCTACATGCAAAATCAGCTGCTCGAAGATTACAAGGAAACGGTACTGGAGCCTTTTCAGGAAAT
GATGCTGTTACAGGTTAAACGTACCTTGCAGTTTTTTTATTCTACCAGCAACCATGGTTTTGTGGATCATATTCTTCTTG
CAGGTGGATTGGCACGGCAGGAAGGACTGGTTGATCTTATCCAGCAACAATTAGGAGTACCTACGACCATTGCAAATCCC
TTTAAACATATGCAATTGGGTAAAATGCTTAACTCGGAAGTCCTCAATAAGGAGGCGCCTGCATTGCTTGTTGCTTGTGG
ATTGGCACTGAGGTCAATAGAGTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A0W0VUE9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	66.384	100	0.664
comM	Acinetobacter baylyi ADP1	42.254	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter nosocomialis M2	42.09	100	0.421