Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   GFX43_RS12455 Genome accession   NZ_CP046448
Coordinates   2446965..2448848 (-) Length   627 a.a.
NCBI ID   WP_015483240.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZD01     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2441965..2453848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFX43_RS12425 (GFX43_012425) fabD 2442553..2443419 (-) 867 WP_015483244.1 ACP S-malonyltransferase -
  GFX43_RS12430 (GFX43_012430) - 2443416..2443682 (-) 267 WP_120363368.1 hypothetical protein -
  GFX43_RS12435 (GFX43_012435) pksB 2443793..2444470 (-) 678 WP_015383701.1 MBL fold metallo-hydrolase -
  GFX43_RS12440 (GFX43_012440) pksA 2444660..2445277 (-) 618 WP_015383700.1 TetR/AcrR family transcriptional regulator -
  GFX43_RS12445 (GFX43_012445) - 2445398..2445955 (+) 558 WP_015483242.1 hypothetical protein -
  GFX43_RS12450 (GFX43_012450) ymzD 2446110..2446565 (+) 456 WP_015483241.1 regulatory YrvL family protein -
  GFX43_RS12455 (GFX43_012455) hexB 2446965..2448848 (-) 1884 WP_015483240.1 DNA mismatch repair endonuclease MutL Machinery gene
  GFX43_RS12460 (GFX43_012460) hexA 2448864..2451440 (-) 2577 WP_015483239.1 DNA mismatch repair protein MutS Machinery gene
  GFX43_RS12465 (GFX43_012465) cotE 2451573..2452118 (-) 546 WP_003231833.1 outer spore coat protein CotE -
  GFX43_RS12470 (GFX43_012470) ymcA 2452380..2452811 (-) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA Regulator

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 70484.35 Da        Isoelectric Point: 5.6008

>NTDB_id=352389 GFX43_RS12455 WP_015483240.1 2446965..2448848(-) (hexB) [Bacillus subtilis strain ZD01]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDADSTVIEIDIEEAGLASIRVLDNGEGMENEDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEITTSTGEGAGTKLVLQGGNIISESRSSSRKGTEIVVSNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHHGKNLLQTNGNGDVRHVLAAIYGTAVAKKMLPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVVNGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHDLIRDG
IKDVFKQQQLIPSAQLPKKSAPVIKNEQQFITFDEKPAEKKVPEKSTASSYSPMKLSSVVKEPLDAEEELPPLQFDAPPI
VDQEQTLEVSDVSAEEPETFEQECHEEQQQPASERVPIMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVG
EVEPEVQDMIVPLTFHYSTNEALIIEQHKQELESVGVFLESFGSNSYIVRCHPAWFPKGEEAELIEEIIQQVLDSKNIDI
KKLREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRSTSDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=352389 GFX43_RS12455 WP_015483240.1 2446965..2448848(-) (hexB) [Bacillus subtilis strain ZD01]
GTGGCAAAAGTCATCCAGCTGTCAGATGAGCTTTCAAATAAAATAGCGGCGGGCGAGGTTGTGGAACGGCCCGCCTCAGT
CGTCAAAGAATTGGTGGAAAATGCGATCGACGCTGACAGCACAGTCATTGAAATCGATATTGAAGAAGCAGGCCTTGCAT
CCATTCGGGTTTTGGATAACGGCGAAGGAATGGAGAATGAAGATTGCAAGCGTGCTTTCCGCCGCCACGCAACGAGTAAA
ATAAAAGATGAAAATGATTTATTCAGGGTGAGAACGCTCGGTTTCAGGGGAGAAGCCCTGCCGAGTATCGCGTCAGTCTC
TCATCTGGAGATTACAACAAGCACTGGTGAAGGAGCTGGGACGAAGCTCGTCCTCCAAGGAGGAAACATCATTTCCGAAT
CGCGTTCCTCAAGCAGGAAGGGAACTGAAATTGTCGTTTCCAACCTGTTTTTTAACACACCGGCCCGCTTGAAATATATG
AAAACCGTTCATACAGAGCTTGGAAACATTACAGATGTGGTGAACCGTATTGCTCTGGCGCATCCAGAGGTATCGATCCG
CCTGCGCCATCATGGGAAAAACCTGCTTCAAACGAACGGAAACGGAGATGTGCGCCATGTACTGGCAGCGATTTACGGCA
CGGCTGTCGCCAAAAAAATGCTCCCGCTGCATGTGAGCTCCTTGGATTTTGAAGTAAAGGGATATATCGCCCTCCCAGAG
ATTACACGGGCGTCGAGAAACTATATGTCGTCTGTGGTCAATGGCCGTTACATTAAAAATTTCCCGCTCGTCAAAGCGGT
TCATGAAGGCTATCATACGCTTCTGCCGATCGGACGCCATCCGATTACTTTTATAGAAATTACGATGGACCCGATTTTAG
TCGATGTCAACGTGCATCCATCGAAGCTTGAGGTCCGGCTCAGCAAGGAAACAGAGCTTCATGACTTAATACGTGACGGA
ATTAAAGATGTGTTTAAACAGCAGCAGCTGATTCCGAGTGCCCAGCTCCCGAAAAAATCGGCACCTGTAATCAAAAATGA
GCAGCAGTTCATAACCTTTGATGAAAAGCCTGCGGAAAAAAAAGTTCCGGAAAAATCGACTGCGTCATCTTATTCACCAA
TGAAGCTCAGTTCAGTCGTAAAAGAGCCGCTTGATGCGGAGGAAGAATTGCCTCCTCTCCAATTTGATGCTCCTCCTATC
GTTGATCAGGAACAAACCCTTGAGGTGTCCGATGTTTCAGCAGAAGAGCCTGAAACATTTGAGCAGGAATGCCATGAGGA
ACAGCAGCAGCCAGCGTCTGAGCGGGTTCCGATTATGTACCCGATCGGCCAGATGCACGGGACTTATATATTGGCACAAA
ACGAAAACGGCCTGTATATTATCGACCAGCACGCCGCCCAAGAACGTATTAAATATGAGTACTTCCGTGAAAAGGTGGGG
GAGGTTGAGCCTGAGGTGCAGGATATGATTGTACCGCTGACGTTCCACTACTCCACAAATGAGGCTTTGATTATTGAACA
GCACAAACAAGAGCTCGAAAGCGTGGGTGTCTTTTTAGAGTCATTCGGCTCTAACAGTTACATCGTCCGCTGCCACCCGG
CCTGGTTTCCAAAGGGAGAAGAAGCAGAGCTCATAGAAGAAATCATTCAGCAGGTGCTCGACTCCAAAAATATAGATATT
AAAAAACTCCGCGAGGAAGCGGCGATTATGATGAGCTGCAAAGGCTCCATCAAAGCAAATCGCCACCTCAGAAACGACGA
AATCAAAGCGCTTCTGGACGACCTCCGAAGCACATCAGACCCATTTACATGCCCGCACGGCCGCCCGATCATCATACACC
ATTCCACTTATGAGATGGAAAAGATGTTCAAACGCGTGATGTAG

Domains

Predicted by InterProScan.

MutL C terminal dimerisation domain (MutL_C)

(442-583)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-324)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

45.652

100

0.469