Detailed information NTG0352390

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: GFX43_RS12460
• Genome accession: NZ_CP046448
• Coordinates: 2448864..2451440 (-)
• Length: 858 a.a.
• NCBI ID: WP_015483239.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain ZD01
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 2443864..2456440

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFX43_RS12440 (GFX43_012440)	pksA	2444660..2445277 (-)	618	WP_015383700.1	TetR/AcrR family transcriptional regulator	-
GFX43_RS12445 (GFX43_012445)	-	2445398..2445955 (+)	558	WP_015483242.1	hypothetical protein	-
GFX43_RS12450 (GFX43_012450)	ymzD	2446110..2446565 (+)	456	WP_015483241.1	regulatory YrvL family protein	-
GFX43_RS12455 (GFX43_012455)	hexB	2446965..2448848 (-)	1884	WP_015483240.1	DNA mismatch repair endonuclease MutL	Machinery gene
GFX43_RS12460 (GFX43_012460)	hexA	2448864..2451440 (-)	2577	WP_015483239.1	DNA mismatch repair protein MutS	Machinery gene
GFX43_RS12465 (GFX43_012465)	cotE	2451573..2452118 (-)	546	WP_003231833.1	outer spore coat protein CotE	-
GFX43_RS12470 (GFX43_012470)	ymcA	2452380..2452811 (-)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
GFX43_RS12475 (GFX43_012475)	miaB	2452813..2454342 (-)	1530	WP_015483238.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
GFX43_RS12480 (GFX43_012480)	kbl	2454490..2455668 (-)	1179	WP_015383694.1	glycine C-acetyltransferase	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97654.73 Da
• Isoelectric Point: 5.1585

>NTDB_id=352390 GFX43_RS12460 WP_015483239.1 2448864..2451440(-) (hexA) [Bacillus subtilis strain ZD01]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFMRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAERIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQQLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPMQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=352390 GFX43_RS12460 WP_015483239.1 2448864..2451440(-) (hexA) [Bacillus subtilis strain ZD01]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTATGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGATG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGGTACAAAGTGGCAATCTGTGAACAAACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTGGTTCAGCT
GATTACGCCTGGAACTGTTATGGACGGCAAAGGCATCCATGAATCGGAAAATAACTTTATCGCATCTGTTTCAGCTTGCT
CGAACGGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACGATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTATGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTATACTCAAAGCGTAATCTGGAGCTGACCGAAACGATCCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATAAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTGATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTATATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTTGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTTCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAGAGCGCATTGACCCTTGCGGGGACGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGTCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGTTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAATTTGCACTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCAGAGCGCTACATTACACCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGTGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTACAGCAGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGTCACTACACGAAACCGGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCAAAAAAAGCGGTGCTTCCGATTTTCGATCAA
ATTTTTACGCGAATCGGTGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAACGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATTAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCACGCGCTCAAGATATTTTAAAAGAGCTTGAGCATTCAGGAAACAAACCGGAAGTGCCGATGCAGAAGCCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

• MutS domain II (MutS_II) (125-239)

• MutS domain I (MutS_I) (5-116)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.591	99.65	0.494
mutS	Pseudomonas stutzeri strain ATCC 17587	39.135	100	0.401