Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   E3O61_RS08130 Genome accession   NZ_CP038166
Coordinates   1666106..1666618 (+) Length   170 a.a.
NCBI ID   WP_002299768.1    Uniprot ID   A0A132P5P8
Organism   Enterococcus faecium strain SC3-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1638926..1673167 1666106..1666618 within 0


Gene organization within MGE regions


Location: 1638926..1673167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3O61_RS08000 (E3O61_08340) - 1638926..1640137 (-) 1212 WP_049143660.1 restriction endonuclease subunit S -
  E3O61_RS08005 (E3O61_08345) - 1640130..1641722 (-) 1593 WP_283651982.1 type I restriction-modification system subunit M -
  E3O61_RS08015 (E3O61_08355) - 1643592..1644317 (-) 726 WP_002332924.1 hypothetical protein -
  E3O61_RS08020 (E3O61_08360) - 1644471..1644797 (-) 327 WP_002332923.1 hypothetical protein -
  E3O61_RS08025 (E3O61_08365) - 1644843..1645205 (-) 363 WP_002324258.1 zinc ribbon domain-containing protein -
  E3O61_RS08030 (E3O61_08370) - 1645242..1645457 (-) 216 WP_002296546.1 helix-turn-helix transcriptional regulator -
  E3O61_RS08035 (E3O61_08375) - 1645585..1645920 (+) 336 WP_002332922.1 helix-turn-helix transcriptional regulator -
  E3O61_RS08040 (E3O61_08380) - 1645994..1648001 (-) 2008 Protein_1531 hypothetical protein -
  E3O61_RS08045 (E3O61_08385) - 1648099..1648373 (-) 275 Protein_1532 helix-turn-helix domain-containing protein -
  E3O61_RS08050 (E3O61_08395) - 1648607..1649323 (+) 717 WP_002324262.1 helix-turn-helix transcriptional regulator -
  E3O61_RS08055 (E3O61_08400) - 1649465..1650628 (+) 1164 WP_002324263.1 site-specific integrase -
  E3O61_RS08060 (E3O61_08405) mnmG 1650705..1652600 (-) 1896 WP_002324264.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  E3O61_RS08065 (E3O61_08410) mnmE 1652617..1654014 (-) 1398 WP_002299773.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  E3O61_RS08070 (E3O61_08415) - 1654314..1654697 (+) 384 WP_002317532.1 hypothetical protein -
  E3O61_RS08075 (E3O61_08420) jag 1654865..1655623 (-) 759 WP_002295258.1 RNA-binding cell elongation regulator Jag/EloR -
  E3O61_RS08080 (E3O61_08425) - 1655641..1656459 (-) 819 WP_002295256.1 YidC/Oxa1 family membrane protein insertase -
  E3O61_RS08085 (E3O61_08430) rnpA 1656456..1656758 (-) 303 WP_283651983.1 ribonuclease P protein component -
  E3O61_RS08090 (E3O61_08435) rpmH 1656848..1656982 (-) 135 WP_002293121.1 50S ribosomal protein L34 -
  E3O61_RS08095 dnaA 1657445..1658428 (+) 984 Protein_1542 chromosomal replication initiator protein DnaA -
  E3O61_RS08100 dnaN 1658411..1659505 (+) 1095 Protein_1543 DNA polymerase III subunit beta -
  E3O61_RS08105 (E3O61_08450) yaaA 1659734..1659976 (+) 243 WP_002295251.1 S4 domain-containing protein YaaA -
  E3O61_RS08110 (E3O61_08455) recF 1659963..1661087 (+) 1125 WP_002288363.1 DNA replication/repair protein RecF -
  E3O61_RS08115 (E3O61_08460) gyrB 1661084..1663030 (+) 1947 WP_002288364.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  E3O61_RS08120 (E3O61_08465) gyrA 1663050..1665521 (+) 2472 WP_002321960.1 DNA gyrase subunit A -
  E3O61_RS08125 (E3O61_08470) rpsF 1665757..1666056 (+) 300 WP_002288366.1 30S ribosomal protein S6 -
  E3O61_RS08130 (E3O61_08475) ssb 1666106..1666618 (+) 513 WP_002299768.1 single-stranded DNA-binding protein Machinery gene
  E3O61_RS08135 (E3O61_08480) rpsR 1666644..1666880 (+) 237 WP_002288370.1 30S ribosomal protein S18 -
  E3O61_RS08140 (E3O61_08485) - 1667023..1668996 (+) 1974 WP_002288371.1 DHH family phosphoesterase -
  E3O61_RS08145 (E3O61_08490) rplI 1669002..1669454 (+) 453 WP_002288372.1 50S ribosomal protein L9 -
  E3O61_RS08150 (E3O61_08495) dnaB 1669728..1671095 (+) 1368 WP_105459697.1 replicative DNA helicase -
  E3O61_RS08155 (E3O61_08505) - 1671875..1673167 (+) 1293 WP_002288375.1 adenylosuccinate synthase -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 18437.96 Da        Isoelectric Point: 4.6742

>NTDB_id=351939 E3O61_RS08130 WP_002299768.1 1666106..1666618(+) (ssb) [Enterococcus faecium strain SC3-1]
MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNREADFINCVIWRKSAETLANYARKGTLVGVTGRIQTR
SYDNQQGQRVYVTEVVADNFQLLESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
IDISDDDLPF

Nucleotide


Download         Length: 513 bp        

>NTDB_id=351939 E3O61_RS08130 WP_002299768.1 1666106..1666618(+) (ssb) [Enterococcus faecium strain SC3-1]
TTGATTAATAATGTTGTATTAGTTGGACGTCTGACGAAAGATCCAGATTTGCGTTACACAGCAAGTGGTACGGCAGTCGC
AACATTTACTTTGGCTGTGAATCGTAATTTTACAAACCAAAGCGGCAATCGCGAGGCTGATTTTATCAACTGTGTGATTT
GGCGCAAATCAGCAGAAACTTTAGCAAACTATGCTCGTAAAGGAACACTTGTAGGTGTTACTGGACGTATCCAAACTCGT
TCTTATGATAATCAGCAAGGACAACGTGTATACGTGACAGAAGTAGTAGCTGACAACTTCCAATTGCTGGAAAGTCGTTC
TGCATCTGAAAATCGCCAACAAAGCGGCGGATTCCAAAGTTCTGGTCAATCAGCTGGCGGATTTGGCGGAAACAATAACT
CTAATCAAACATCTCAATCATCCAACGGTATGCCTGATTTCGACCGTGATACTTCTGACCCATTCGGCTCAAGTTCAACG
ATCGACATTTCAGACGATGATTTACCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A132P5P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.887

100

0.624
  ssbA Bacillus subtilis subsp. subtilis str. 168

56.983

100

0.6
  ssbB Bacillus subtilis subsp. subtilis str. 168

63.208

62.353

0.394

Multiple sequence alignment