Detailed information NTG0351659

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E3U44_RS11860
• Genome accession: NZ_CP038033
• Coordinates: 2457067..2458239 (-)
• Length: 390 a.a.
• NCBI ID: WP_134358396.1
• Uniprot ID: A0A4P7BY75
• Organism: Nitrosococcus wardiae strain D1FHS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2452067..2463239

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U44_RS11840 (E3U44_11840)	-	2452120..2454801 (-)	2682	WP_134358392.1	bifunctional acetate--CoA ligase family protein/GNAT family N-acetyltransferase	-
E3U44_RS11845 (E3U44_11845)	-	2455064..2455516 (-)	453	WP_134358393.1	HU family DNA-binding protein	-
E3U44_RS11850 (E3U44_11850)	-	2455896..2456363 (-)	468	WP_134358394.1	hypothetical protein	-
E3U44_RS11855 (E3U44_11855)	-	2456531..2456860 (-)	330	WP_134358395.1	DMT family transporter	-
E3U44_RS11860 (E3U44_11860)	pilU	2457067..2458239 (-)	1173	WP_134358396.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E3U44_RS11865 (E3U44_11865)	-	2458379..2459293 (+)	915	WP_134358397.1	AEC family transporter	-
E3U44_RS11870 (E3U44_11870)	-	2459304..2460050 (-)	747	WP_134358398.1	competence/damage-inducible protein A	-
E3U44_RS11875 (E3U44_11875)	-	2460187..2461344 (-)	1158	WP_134359818.1	RNA-guided endonuclease InsQ/TnpB family protein	-
E3U44_RS11880 (E3U44_11880)	-	2461391..2461531 (+)	141	WP_240761824.1	ribbon-helix-helix protein, CopG family	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43086.14 Da
• Isoelectric Point: 6.5592

>NTDB_id=351659 E3U44_RS11860 WP_134358396.1 2457067..2458239(-) (pilU) [Nitrosococcus wardiae strain D1FHS]
MEFKDYLKILVAHDGSDVYLSAGAPPCAKFHGLLKPLEKKALTSNDIKEIAYGIMNEEQTAEFENKPEMNLAISETGIGR
FRVNIFKQRSAVSMVIRNIKTTIPNADNLGLPPILKEVVMSKHGLILFVGATGSGKSTSLAALIDHRNNHSAGHIITIED
PIEYIHHHKKSIINQREVGVDTDCYEDALKNTLRQAPDVILIGEIRDQNTMEHAIAFAETGHLCLSTLHANNANQALDRI
INFFPEDRRSQLLLDLSLNLRAFVSQRLVPTIDGKRTAAIEILLGTPLVRDLIHRGDIHAIKEVMEKSENLGMQTFDSAL
YKLYITGHISVEEALRNADSPNNLRLKINLSKKNPGATAKPTSTENASSEQKTEKSASGGLSFSLVEEED

Nucleotide

• Length: 1173 bp

>NTDB_id=351659 E3U44_RS11860 WP_134358396.1 2457067..2458239(-) (pilU) [Nitrosococcus wardiae strain D1FHS]
ATGGAATTTAAGGATTACCTGAAAATATTAGTGGCCCACGATGGCTCGGATGTTTATCTCAGTGCCGGAGCCCCCCCTTG
TGCCAAGTTTCACGGTTTACTGAAACCCCTTGAAAAAAAAGCCTTAACCTCTAACGACATTAAGGAAATAGCCTATGGCA
TCATGAATGAAGAACAAACAGCCGAGTTTGAGAACAAACCTGAGATGAATTTAGCCATTTCCGAAACGGGCATAGGACGC
TTTCGGGTCAATATTTTCAAGCAGCGCAGTGCCGTTTCGATGGTCATCCGAAATATTAAGACCACTATCCCCAACGCGGA
TAACCTCGGTCTACCCCCCATATTAAAAGAGGTTGTCATGTCTAAACATGGCCTCATTCTGTTTGTAGGGGCAACGGGTT
CCGGCAAATCTACTTCCCTTGCCGCGCTCATCGACCACCGCAATAATCACAGCGCCGGGCATATCATCACCATTGAGGAC
CCTATCGAGTATATCCATCACCATAAAAAATCTATTATTAATCAACGGGAAGTGGGCGTTGACACGGATTGCTATGAAGA
TGCGCTTAAAAACACCCTTCGCCAAGCTCCTGATGTGATTCTGATTGGAGAAATCCGTGATCAGAACACGATGGAACATG
CCATTGCCTTTGCAGAAACAGGCCATTTGTGCCTCTCAACCCTGCATGCTAACAATGCCAACCAAGCCCTTGACCGGATC
ATTAACTTTTTCCCCGAAGACCGGCGTTCGCAGCTTCTATTGGACCTCTCCTTGAACCTACGGGCTTTTGTGTCTCAACG
TCTCGTTCCCACTATTGATGGCAAACGCACCGCGGCTATTGAAATTTTACTGGGTACGCCTTTGGTTCGCGATCTTATCC
ACCGCGGTGATATCCATGCCATTAAAGAAGTAATGGAAAAGTCAGAAAATCTCGGCATGCAAACTTTTGACAGTGCCCTC
TATAAATTATATATAACAGGCCATATCAGCGTTGAGGAAGCATTACGCAATGCCGATTCCCCTAACAACCTCCGCCTTAA
AATCAACTTATCAAAGAAAAACCCCGGGGCTACCGCTAAGCCCACCTCCACAGAAAACGCTTCCTCAGAGCAAAAAACGG
AAAAGAGCGCATCAGGGGGACTCTCTTTTAGCCTAGTGGAAGAAGAAGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-269)

3D structure

• AlphaFold DB: A0A4P7BY75

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.543	91.538	0.536
pilU	Acinetobacter baylyi ADP1	54.331	97.692	0.531
pilU	Vibrio cholerae strain A1552	49.575	90.513	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.192	98.974	0.408