Detailed information NTG0035162

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CAP2UW1_RS20715
• Genome accession: NC_013194
• Coordinates: 4737616..4738476 (-)
• Length: 286 a.a.
• NCBI ID: WP_015768570.1
• Uniprot ID: A0A9D8KRI8
• Organism: Candidatus Accumulibacter phosphatis
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4732616..4743476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CAP2UW1_RS26260	-	4733237..4733449 (+)	213	WP_081444240.1	cysteine-rich CWC family protein	-
CAP2UW1_RS20685 (CAP2UW1_4161)	nudB	4733412..4733867 (-)	456	WP_015768564.1	dihydroneopterin triphosphate diphosphatase	-
CAP2UW1_RS24180 (CAP2UW1_4162)	-	4733864..4734307 (-)	444	WP_015768565.1	barstar family protein	-
CAP2UW1_RS20695 (CAP2UW1_4163)	-	4734304..4734711 (-)	408	WP_015768566.1	ribonuclease domain-containing protein	-
CAP2UW1_RS20700 (CAP2UW1_4164)	aspS	4734718..4736520 (-)	1803	WP_015768567.1	aspartate--tRNA ligase	-
CAP2UW1_RS20705 (CAP2UW1_4165)	-	4736544..4737191 (-)	648	WP_015768568.1	DUF502 domain-containing protein	-
CAP2UW1_RS20710 (CAP2UW1_4166)	-	4737176..4737508 (-)	333	WP_015768569.1	FmdB family zinc ribbon protein	-
CAP2UW1_RS20715 (CAP2UW1_4167)	pilD	4737616..4738476 (-)	861	WP_015768570.1	prepilin peptidase	Machinery gene
CAP2UW1_RS20720 (CAP2UW1_4168)	pilC	4738476..4739714 (-)	1239	WP_015768571.1	type II secretion system F family protein	Machinery gene
CAP2UW1_RS20725 (CAP2UW1_4169)	pilB	4739737..4741452 (-)	1716	WP_015768572.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CAP2UW1_RS26265 (CAP2UW1_4170)	-	4741638..4741766 (-)	129	WP_291911647.1	hypothetical protein	-
CAP2UW1_RS20730 (CAP2UW1_4171)	xerC	4741877..4742791 (-)	915	WP_015768574.1	tyrosine recombinase XerC	-
CAP2UW1_RS20735 (CAP2UW1_4172)	-	4742802..4743455 (-)	654	WP_015768575.1	DUF484 family protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 30836.78 Da
• Isoelectric Point: 8.2923

>NTDB_id=35162 CAP2UW1_RS20715 WP_015768570.1 4737616..4738476(-) (pilD) [Candidatus Accumulibacter phosphatis]
MPGAPAVFADPLLFALLSGVVGLLVGSFLNVVIYRLPKMMDRDWRVHCAELRGEVLPEQERFSLAWPGSRCPACGHAITA
MENVPVLSWLFLRGRCSACRQPISSRYPLVEAISGLLCAFAAFHFGYGWAAAGAMLLIWCLIALTCIDLDTLLLPDSITL
PLLWAGLLFNLFGTFADLHSAVIGAASGYLSLWLVYWGFKLTTGKEGMGYGDFKLLAALGAWFGWQMLPLTILLSSLLGA
VVGIAMIVVAKRGRSVPIPFGPYLAAAGGLALFWGKPLTRAYVGLL

Nucleotide

• Length: 861 bp

>NTDB_id=35162 CAP2UW1_RS20715 WP_015768570.1 4737616..4738476(-) (pilD) [Candidatus Accumulibacter phosphatis]
ATGCCGGGCGCGCCGGCCGTCTTTGCGGACCCGCTGCTGTTCGCTCTCCTCAGCGGCGTCGTCGGACTGCTGGTCGGCAG
TTTCCTCAATGTCGTCATCTATCGCCTGCCAAAGATGATGGATCGCGATTGGCGTGTCCATTGCGCCGAGCTGCGGGGCG
AGGTGTTGCCGGAGCAGGAGCGGTTTTCGCTGGCCTGGCCCGGCTCGCGTTGCCCTGCTTGCGGGCACGCCATCACGGCC
ATGGAGAATGTTCCTGTCCTGAGCTGGCTCTTCTTGCGCGGCCGCTGTTCGGCGTGTCGGCAGCCGATATCGTCGCGCTA
TCCGCTGGTCGAGGCGATCAGCGGCCTGCTGTGCGCTTTCGCCGCGTTTCATTTCGGCTATGGCTGGGCGGCGGCGGGCG
CCATGTTGTTGATCTGGTGCCTGATCGCCCTGACCTGCATCGATCTCGACACGTTGTTGCTGCCTGACTCGATTACCTTG
CCCCTGCTGTGGGCCGGCCTCCTGTTCAACCTCTTCGGGACTTTCGCCGACCTGCACTCGGCGGTGATCGGCGCCGCCAG
CGGCTATCTCTCGCTCTGGCTGGTCTACTGGGGCTTCAAGTTGACCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCA
AGCTGCTCGCTGCCCTCGGTGCCTGGTTCGGTTGGCAGATGCTGCCTCTGACGATCCTGCTGTCGTCCCTGTTGGGCGCC
GTTGTCGGCATCGCAATGATCGTCGTCGCCAAACGCGGCCGCAGCGTGCCGATCCCGTTTGGTCCCTATCTGGCGGCAGC
AGGCGGGCTTGCCTTGTTCTGGGGCAAACCGCTCACCCGCGCCTACGTCGGCCTGCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.455	96.154	0.514
pilD	Vibrio campbellii strain DS40M4	51.636	96.154	0.497
pilD	Acinetobacter baumannii D1279779	50.746	93.706	0.476
pilD	Acinetobacter nosocomialis M2	49.627	93.706	0.465
pilD	Neisseria gonorrhoeae MS11	51.575	88.811	0.458