Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GNX12_RS03530 Genome accession   NZ_CP046396
Coordinates   749673..750332 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain ampC_0069     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 744673..755332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX12_RS03500 (GNX12_03505) - 744789..745736 (+) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -
  GNX12_RS03505 (GNX12_03510) - 745733..746620 (+) 888 WP_000614960.1 MurR/RpiR family transcriptional regulator -
  GNX12_RS03510 (GNX12_03515) ygiN 746665..746979 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  GNX12_RS03515 (GNX12_03520) mdaB 747010..747591 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GNX12_RS03520 (GNX12_03525) ygiZ 747949..748281 (+) 333 WP_022646136.1 DUF2645 family protein -
  GNX12_RS03525 (GNX12_03530) qseC 748327..749676 (-) 1350 WP_022646135.1 quorum sensing histidine kinase QseC -
  GNX12_RS03530 (GNX12_03535) ciaR 749673..750332 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GNX12_RS03535 (GNX12_03540) ygiW 750484..750876 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GNX12_RS03540 (GNX12_03545) ygiV 750929..751411 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  GNX12_RS03545 (GNX12_03550) ygiS 751520..753127 (+) 1608 WP_022646134.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=351554 GNX12_RS03530 WP_001221493.1 749673..750332(-) (ciaR) [Escherichia coli strain ampC_0069]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=351554 GNX12_RS03530 WP_001221493.1 749673..750332(-) (ciaR) [Escherichia coli strain ampC_0069]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment