Detailed information NTG0350301

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HPF_RS19660
• Genome accession: NZ_CP037867
• Coordinates: 4086143..4086985 (+)
• Length: 280 a.a.
• NCBI ID: WP_133157604.1
• Uniprot ID: A0A4P6X852
• Organism: Hydrogenophaga pseudoflava strain DSM 1084
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4081143..4091985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPF_RS19635 (HPF_19510)	rplU	4081351..4081662 (-)	312	WP_066152961.1	50S ribosomal protein L21	-
HPF_RS19640 (HPF_19515)	-	4081945..4082874 (+)	930	WP_133158174.1	polyprenyl synthetase family protein	-
HPF_RS19650 (HPF_19525)	pilB	4083153..4084889 (+)	1737	WP_133157603.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HPF_RS19655 (HPF_19530)	pilC	4084920..4086137 (+)	1218	WP_066152952.1	type II secretion system F family protein	Machinery gene
HPF_RS19660 (HPF_19535)	pilD	4086143..4086985 (+)	843	WP_133157604.1	A24 family peptidase	Machinery gene
HPF_RS19665 (HPF_19540)	coaE	4087000..4087590 (+)	591	WP_133157605.1	dephospho-CoA kinase	-
HPF_RS19670 (HPF_19545)	zapD	4087631..4088386 (+)	756	WP_066152938.1	cell division protein ZapD	-
HPF_RS19675 (HPF_19550)	-	4088395..4088583 (+)	189	WP_066152933.1	DNA gyrase inhibitor YacG	-
HPF_RS19680 (HPF_19555)	-	4088590..4089060 (-)	471	WP_066152930.1	NUDIX domain-containing protein	-
HPF_RS19685 (HPF_19560)	-	4089057..4089935 (-)	879	WP_066152927.1	ATP-binding protein	-
HPF_RS19690 (HPF_19565)	argJ	4089952..4091199 (-)	1248	WP_066152924.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 29849.28 Da
• Isoelectric Point: 5.6491

>NTDB_id=350301 HPF_RS19660 WP_133157604.1 4086143..4086985(+) (pilD) [Hydrogenophaga pseudoflava strain DSM 1084]
MVNLLDAALLGVLGLLVGSFLNVVIHRLPKMMELRWAAECAELQGTATDTDAAAPYNLMVPRSACPHCGHQIRWFENVPV
VSYLALGGKCAGCKAPISLRYPTVELVASALFAWCGWTWGLGWEALVWSGFAASLVTLTCIDWDTTLLPDDITLPLLWAG
LCASALGFTGVSLAEALWGAVGGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILMASVIGAIVGIG
IKMFSQLREGGYVPFGPFLALAGLTSLFFGPRAILAAIGL

Nucleotide

• Length: 843 bp

>NTDB_id=350301 HPF_RS19660 WP_133157604.1 4086143..4086985(+) (pilD) [Hydrogenophaga pseudoflava strain DSM 1084]
ATGGTCAACCTGCTGGACGCTGCACTTCTGGGCGTTCTGGGGCTGCTGGTCGGCAGCTTCCTCAATGTGGTCATCCACCG
ACTGCCCAAGATGATGGAGTTGCGGTGGGCCGCCGAATGTGCAGAACTGCAAGGCACAGCAACAGACACTGATGCTGCGG
CCCCCTACAACCTCATGGTGCCTCGCTCTGCCTGCCCGCATTGCGGACACCAGATCCGGTGGTTTGAAAATGTTCCGGTG
GTCAGCTATCTTGCCCTTGGAGGCAAATGCGCCGGCTGCAAAGCGCCCATCAGTCTCCGCTACCCGACAGTGGAGCTCGT
TGCGAGCGCTCTGTTCGCCTGGTGCGGCTGGACATGGGGGCTGGGTTGGGAGGCGTTGGTCTGGAGCGGGTTCGCCGCAA
GCCTAGTGACACTGACCTGCATTGACTGGGACACCACACTGCTGCCCGACGACATTACCTTGCCGCTGCTTTGGGCTGGC
TTGTGCGCTTCAGCGCTTGGCTTCACCGGCGTGTCGCTGGCCGAAGCGCTGTGGGGAGCTGTCGGGGGCTATCTGTCACT
TTGGCTGGTGTATTGGGGATTCAAGCTGGCGACGGGCAAGGAAGGCATGGGCTACGGCGATTTCAAGCTCTTCGCCGCGC
TGGGAGCATGGTTCGGCTGGCAGGCACTCGTGCCGATCATCCTCATGGCATCGGTCATCGGTGCCATCGTTGGCATTGGC
ATCAAGATGTTTTCCCAACTGCGCGAAGGAGGCTATGTTCCGTTTGGCCCCTTCCTGGCACTGGCCGGACTCACTTCGCT
GTTCTTCGGGCCCCGCGCCATTCTGGCCGCGATTGGCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (132-239)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (12-118)

3D structure

• AlphaFold DB: A0A4P6X852

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.746	95.714	0.486
pilD	Vibrio campbellii strain DS40M4	50	94.286	0.471
pilD	Neisseria gonorrhoeae MS11	47.843	91.071	0.436
pilD	Acinetobacter baumannii D1279779	42.804	96.786	0.414
pilD	Acinetobacter nosocomialis M2	42.066	96.786	0.407