Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GJD96_RS05130 Genome accession   NZ_CP046009
Coordinates   724468..725127 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 1919D62     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 719468..730127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD96_RS05105 (GJD96_05110) - 720124..721455 (+) 1332 WP_000778881.1 MFS transporter -
  GJD96_RS05110 (GJD96_05115) ygiN 721499..721813 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  GJD96_RS05115 (GJD96_05120) mdaB 721844..722425 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GJD96_RS05120 (GJD96_05125) ygiZ 722744..723076 (+) 333 WP_096310111.1 DUF2645 family protein -
  GJD96_RS05125 (GJD96_05130) qseC 723122..724471 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  GJD96_RS05130 (GJD96_05135) ciaR 724468..725127 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GJD96_RS05135 (GJD96_05140) ygiW 725279..725671 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GJD96_RS05140 (GJD96_05145) ygiV 725724..726206 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  GJD96_RS05145 (GJD96_05150) parC 726752..729010 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  GJD96_RS05150 (GJD96_05155) plsC 729244..729981 (+) 738 WP_000965722.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=349515 GJD96_RS05130 WP_001221493.1 724468..725127(-) (ciaR) [Escherichia coli strain 1919D62]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=349515 GJD96_RS05130 WP_001221493.1 724468..725127(-) (ciaR) [Escherichia coli strain 1919D62]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365