Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   EZZ25_RS09010 Genome accession   NZ_CP036499
Coordinates   1873122..1875365 (+) Length   747 a.a.
NCBI ID   WP_165490556.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1868122..1880365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS08985 (EZZ25_08985) - 1868585..1869160 (-) 576 WP_000999604.1 PilZ domain-containing protein -
  EZZ25_RS08990 (EZZ25_08990) lolC 1869336..1870544 (+) 1209 WP_165490529.1 lipoprotein-releasing ABC transporter permease subunit LolC -
  EZZ25_RS08995 (EZZ25_08995) lolD 1870537..1871223 (+) 687 WP_001061290.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  EZZ25_RS09000 (EZZ25_09000) lolE 1871224..1872468 (+) 1245 WP_000493012.1 lipoprotein-releasing ABC transporter permease subunit LolE -
  EZZ25_RS09005 (EZZ25_09005) - 1872583..1873113 (-) 531 WP_001881633.1 DUF2062 domain-containing protein -
  EZZ25_RS09010 (EZZ25_09010) comEC 1873122..1875365 (+) 2244 WP_165490556.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EZZ25_RS09015 (EZZ25_09015) msbA 1875396..1877144 (+) 1749 WP_000052155.1 lipid A ABC transporter ATP-binding protein/permease MsbA -
  EZZ25_RS09020 (EZZ25_09020) lpxK 1877147..1878154 (+) 1008 WP_032481777.1 tetraacyldisaccharide 4'-kinase -
  EZZ25_RS09025 (EZZ25_09025) - 1878135..1878314 (+) 180 WP_000350068.1 Trm112 family protein -
  EZZ25_RS09030 (EZZ25_09030) kdsB 1878314..1879072 (+) 759 WP_000011327.1 3-deoxy-manno-octulosonate cytidylyltransferase -

Sequence

Protein


Download         Length: 747 a.a.        Molecular weight: 84371.51 Da        Isoelectric Point: 7.9710

>NTDB_id=349237 EZZ25_RS09010 WP_165490556.1 1873122..1875365(+) (comEC) [Vibrio cholerae strain 20000]
MTLLSNYWSLISFSITVLSAPYWPWMPSWGWAWLCLIVMVLLGYHRVGRQFLGFVAAILTIVLQGNLIRDQSNVLYQAGP
DIIIKGRVDSFFTQTRYAYEGFVLIHEVNGQTLNKMTRPRIRLSAPLLLQPNDRVEFSVTLKPIVGRLNQTGFDLEAHYM
AQSVVARAVVKPDTAYQIVQESGIRSSLFFELEQLTHTSPYQGLILALTFGERKGIDEQEWQALRNSGLIHLVAISGLHI
GIAFSVGYFLGLGMMRLHAQLLWSPFVCGALLAVLYAWLAGFTLPTQRALIMCLLNVALIMLAFPLSALKRILLTLGAVL
LWSPFASLSNSFWMSFLAVAIVLYQLASQSQRQVWWKALLWAQVFLVCLMAPVTAYFFGGLSVTAVLYNLVFIPWFSLVI
VPALFLGLLLMVVWPSVAAAYWPWVDWTFLPLDWALQFADVGWWVVPSKVQGVVAASVAILLLYRFMSLKACSLLLGMIG
LWWWFPSLTPLWRMDVLDVGHGLAIVIEQDERAIVYDTGSSWPGGSYVQSVIEPILQQRGLRQVDGVILSHLDNDHAGDW
QGLAERWQPNWIRASQLGTEFMPCIRGESWQWQSLHFTVLWPPQAVSRAYNQHSCVIRMTDTQSNHSVLLSGDVTAMGEW
LLARDGAQLQSEVMIVPHHGSKTSSTAEFIAQVNPKLAIASVAKDNRWNLPNPQVVARYQAQQVEWLDTGHAGQISLFFY
PDQLDWFTQRSLGWQPRYRQMLRKGVE

Nucleotide


Download         Length: 2244 bp        

>NTDB_id=349237 EZZ25_RS09010 WP_165490556.1 1873122..1875365(+) (comEC) [Vibrio cholerae strain 20000]
ATGACTCTCTTGTCGAATTACTGGTCTCTCATTTCTTTTTCGATCACCGTGCTGTCTGCCCCTTATTGGCCTTGGATGCC
GAGTTGGGGCTGGGCTTGGTTATGCCTTATTGTTATGGTTTTGCTCGGTTATCACCGAGTTGGCCGTCAATTCCTTGGCT
TCGTGGCTGCCATACTAACCATTGTGCTACAGGGCAACCTTATACGAGATCAATCCAATGTGCTCTATCAAGCAGGGCCG
GATATTATCATAAAAGGCCGTGTTGACAGCTTTTTTACGCAAACTCGTTACGCTTATGAGGGTTTTGTCCTCATTCATGA
AGTGAATGGACAAACCTTAAACAAAATGACTCGCCCTCGCATACGTTTAAGTGCCCCTTTACTGTTACAACCCAATGACC
GCGTCGAATTTTCGGTAACTCTCAAGCCGATAGTGGGTCGACTCAACCAAACCGGCTTTGATTTAGAAGCGCATTACATG
GCGCAATCTGTCGTCGCACGGGCGGTCGTAAAACCTGACACCGCTTATCAAATTGTGCAAGAGAGTGGCATAAGGTCAAG
TTTGTTTTTTGAGCTGGAACAATTAACGCATACCAGCCCATACCAAGGATTGATCTTAGCCCTGACGTTTGGCGAGCGAA
AAGGTATTGATGAGCAAGAGTGGCAAGCCTTACGCAATAGTGGCTTAATTCATTTAGTGGCGATTTCGGGGCTGCACATT
GGTATCGCTTTTAGCGTGGGTTATTTTCTCGGGCTCGGCATGATGCGTCTTCATGCTCAGTTATTGTGGTCCCCTTTTGT
GTGTGGGGCTTTACTGGCGGTGCTCTACGCTTGGCTGGCTGGATTTACGTTGCCTACTCAGCGTGCATTGATTATGTGCT
TACTCAATGTGGCGTTGATCATGTTGGCTTTTCCTCTTTCCGCGCTCAAGCGGATTCTACTCACCTTGGGCGCGGTCTTG
CTTTGGTCGCCATTCGCCTCACTTTCAAACAGTTTCTGGATGTCGTTTTTGGCGGTCGCGATTGTTCTCTACCAATTAGC
CAGTCAAAGCCAGCGTCAGGTGTGGTGGAAAGCTCTGCTTTGGGCGCAGGTGTTCCTCGTCTGTTTAATGGCACCGGTCA
CGGCCTATTTTTTCGGTGGCTTAAGCGTAACGGCAGTTCTGTACAATTTGGTGTTTATTCCTTGGTTTTCGTTGGTGATT
GTCCCAGCTTTGTTTTTGGGCCTACTACTCATGGTGGTATGGCCTAGTGTGGCCGCCGCTTACTGGCCTTGGGTGGATTG
GACGTTTTTACCGCTCGATTGGGCTTTGCAGTTTGCCGATGTAGGCTGGTGGGTGGTCCCCAGCAAAGTACAAGGTGTAG
TCGCAGCGAGTGTGGCCATCCTCTTGCTTTATCGATTTATGAGCCTAAAAGCCTGCAGCTTATTATTGGGTATGATTGGC
TTATGGTGGTGGTTTCCCTCTCTCACTCCACTTTGGCGAATGGATGTGCTGGATGTTGGACATGGCTTGGCGATTGTGAT
TGAGCAAGATGAGCGAGCAATTGTCTACGATACAGGCAGCAGTTGGCCAGGAGGCAGCTATGTGCAAAGCGTGATTGAGC
CTATTCTCCAACAGCGAGGGCTACGCCAAGTCGATGGAGTGATTTTAAGTCATCTTGATAATGATCATGCGGGTGATTGG
CAAGGTTTAGCTGAGCGCTGGCAACCTAATTGGATTCGTGCCAGCCAACTCGGGACAGAGTTTATGCCTTGTATCCGTGG
TGAAAGCTGGCAGTGGCAATCTCTCCATTTTACGGTGTTATGGCCACCACAAGCGGTTAGCCGAGCGTACAACCAGCATT
CGTGTGTGATTCGTATGACTGATACTCAGTCTAACCATTCTGTACTGCTCTCCGGGGATGTCACAGCCATGGGGGAGTGG
CTGCTTGCTCGCGACGGAGCGCAACTGCAAAGTGAGGTGATGATCGTGCCGCACCACGGCAGTAAAACGTCGTCCACCGC
AGAGTTTATTGCCCAAGTGAATCCCAAACTTGCGATTGCTTCTGTGGCGAAAGATAACCGCTGGAATTTGCCTAATCCGC
AAGTCGTGGCACGTTATCAAGCTCAGCAAGTTGAGTGGCTAGATACTGGACACGCTGGGCAAATTAGCCTCTTTTTCTAT
CCAGATCAGCTGGATTGGTTTACCCAGCGTAGCCTTGGCTGGCAGCCTCGGTATAGGCAGATGCTGCGTAAAGGAGTAGA
ATGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(21-173)

Competence protein (Competence)

(208-464)

Metallo-beta-lactamase superfamily (Lactamase_B)

(499-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Vibrio cholerae strain A1552

99.331

100

0.993
  comEC Vibrio parahaemolyticus RIMD 2210633

41.215

100

0.418
  comEC Vibrio campbellii strain DS40M4

40.957

100

0.412

Multiple sequence alignment