Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GIY05_RS28750 Genome accession   NZ_CP045916
Coordinates   6171904..6172527 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CF39S     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6166904..6177527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIY05_RS28730 aroB 6167499..6168605 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  GIY05_RS28735 aroK 6168655..6169173 (-) 519 WP_003095833.1 shikimate kinase AroK -
  GIY05_RS28740 pilQ 6169185..6171329 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  GIY05_RS28745 comL/pilP 6171383..6171907 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  GIY05_RS28750 pilO 6171904..6172527 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  GIY05_RS28755 pilN 6172524..6173120 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  GIY05_RS28760 pilM 6173120..6174184 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  GIY05_RS28765 - 6174369..6176840 (+) 2472 WP_003103276.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=348505 GIY05_RS28750 WP_003103268.1 6171904..6172527(-) (pilO) [Pseudomonas aeruginosa strain CF39S]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=348505 GIY05_RS28750 WP_003103268.1 6171904..6172527(-) (pilO) [Pseudomonas aeruginosa strain CF39S]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401

Multiple sequence alignment