Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   GII77_RS09255 Genome accession   NZ_CP045825
Coordinates   1745738..1747786 (+) Length   682 a.a.
NCBI ID   WP_015252106.1    Uniprot ID   -
Organism   Bacillus subtilis strain 75     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1740738..1752786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII77_RS09225 (GII77_09225) spoVM 1741374..1741454 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  GII77_RS09230 (GII77_09230) rpmB 1741527..1741715 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  GII77_RS09235 (GII77_09235) yloU 1741992..1742354 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  GII77_RS09240 (GII77_09240) fakA 1742370..1744031 (+) 1662 WP_015252107.1 DAK2 domain-containing protein -
  GII77_RS09245 (GII77_09245) sdaAB 1744170..1744832 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  GII77_RS09250 (GII77_09250) sdaAA 1744858..1745760 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  GII77_RS09255 (GII77_09255) recG 1745738..1747786 (+) 2049 WP_015252106.1 ATP-dependent DNA helicase RecG Machinery gene
  GII77_RS09260 (GII77_09260) fapR 1747895..1748461 (+) 567 WP_003232044.1 transcription factor FapR -
  GII77_RS09265 (GII77_09265) plsX 1748475..1749476 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  GII77_RS09270 (GII77_09270) fabD 1749495..1750448 (+) 954 WP_003245314.1 ACP S-malonyltransferase -
  GII77_RS09275 (GII77_09275) fabG 1750441..1751181 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  GII77_RS09280 (GII77_09280) acpP 1751265..1751498 (+) 234 WP_003154310.1 acyl carrier protein -
  GII77_RS09285 (GII77_09285) rncS 1751638..1752387 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78140.68 Da        Isoelectric Point: 7.2507

>NTDB_id=347865 GII77_RS09255 WP_015252106.1 1745738..1747786(+) (recG) [Bacillus subtilis strain 75]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKQLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=347865 GII77_RS09255 WP_015252106.1 1745738..1747786(+) (recG) [Bacillus subtilis strain 75]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTTGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATTACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGCAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGGTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACGTTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATCGATGAGATGCCGGCTGGACGAAAGAGAATCGAAACCTATTGGGTAAAACATGACATGCTGGATC
GTATATTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAC
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGGCTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAAACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

DEAD/DEAH box helicase (DEAD)

(260-415)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.853

100

0.999
  recG/mmsA Streptococcus pneumoniae R6

48.82

99.413

0.485
  recG/mmsA Streptococcus pneumoniae R36A

48.82

99.413

0.485
  recG Neisseria meningitidis strain C311

39.695

96.041

0.381