Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdL   Type   Machinery gene
Locus tag   RJ40_RS04320 Genome accession   NZ_CP036172
Coordinates   888334..890685 (-) Length   783 a.a.
NCBI ID   WP_265582129.1    Uniprot ID   A0A8A3S4B9
Organism   Methanofollis aquaemaris strain N2F9704     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883334..895685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ40_RS04295 (RJ40_04245) - 883374..883718 (-) 345 WP_265582125.1 type II secretion system protein E -
  RJ40_RS04300 (RJ40_04250) - 883756..885240 (-) 1485 WP_265582605.1 cobyric acid synthase -
  RJ40_RS04305 (RJ40_04255) yciH 885410..885715 (+) 306 WP_265582126.1 stress response translation initiation inhibitor YciH -
  RJ40_RS04310 (RJ40_04260) epdK 886375..887412 (-) 1038 WP_265582127.1 type II secretion system F family protein Machinery gene
  RJ40_RS04315 (RJ40_04265) epdJ 887414..888337 (-) 924 WP_265582128.1 type II secretion system F family protein Machinery gene
  RJ40_RS04320 (RJ40_04270) epdL 888334..890685 (-) 2352 WP_265582129.1 type II/IV secretion system ATPase subunit Machinery gene
  RJ40_RS04325 (RJ40_04275) - 890678..891304 (-) 627 WP_265582130.1 hypothetical protein -
  RJ40_RS04330 (RJ40_04280) minD 891412..892197 (-) 786 WP_265582131.1 cell division ATPase MinD -
  RJ40_RS04335 (RJ40_04285) - 892323..892571 (-) 249 WP_265582132.1 hypothetical protein -
  RJ40_RS04340 (RJ40_04290) - 892922..893671 (-) 750 WP_265582133.1 roadblock/LC7 domain-containing protein -
  RJ40_RS04345 (RJ40_04295) - 893674..894021 (-) 348 WP_322743911.1 roadblock/LC7 domain-containing protein -
  RJ40_RS04350 (RJ40_04300) - 894030..895433 (-) 1404 WP_265582135.1 Ni/Fe hydrogenase subunit alpha -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 87770.73 Da        Isoelectric Point: 4.5916

>NTDB_id=347321 RJ40_RS04320 WP_265582129.1 888334..890685(-) (epdL) [Methanofollis aquaemaris strain N2F9704]
MSEKQRPYRGLHDILERMKGSENKKEKQRDPPKTEKKDETSGLRSLIGKKDGLDLSALVRDPRNYGLGEEKPAAPPGTEE
PADTPIAETPHHEPQDEEFDDQAIRELKSAVDAILSAREDEAPAPTLSMEEETPLEEEAVSPAPEAVPVSRKSRWSSEVG
NPEEEPGADITDVDDLSVYSDLILPKAATFDIEEFTLQRPNHRETLGVNNLPPEIDVLWADTVPTIAEEARNVVIGREKG
EKSGFLGKIGGIGKIGAFDRTQHKSSVEEYDPAVHGPLVDLTFTPPAGVEEVELYPVNEPFAYVRISYDSTSHEYSYEVL
EPALTAAENELFGEIKDRLFERLDVSTKDLSREGARQILRDLSDEIVADYGIHLSSLSREKIYYTIERDFLGNGLIDAIM
HDKYIEDISCDGLDNPVFVFHSGYESIKTNLMYTNAVELDSFVTKLAQRAGKYISIADPILDATMSDGSRIQMTLGKEVT
AHGSTFTIRKFKDEPITPTDLIGWGTFSPLSVAFLWLAVEAGNSAIFAGGTASGKTTALNAISLFIPPQAKIVSLEDTRE
LKLPHANWIPSVTRESFDTEGKGTIDMYELLRAALRQRPEYMCVGEVRGAEAQTLFQAMSTGHVTYATMHADSVASVVHR
LENPPLDVPRNMLNALNLVCIQAQARIGGQRIRRNKQIIEILDIDPRTNELITNEVFSWHQATDEIRYSGKSYILETIME
TKGWSEARMREELKRRQEMLEWMRVKNIRHYKDVSKMLISYFRDADAVIGLVREELYREADQQ

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=347321 RJ40_RS04320 WP_265582129.1 888334..890685(-) (epdL) [Methanofollis aquaemaris strain N2F9704]
ATGTCTGAGAAACAGAGACCCTACAGAGGTCTTCATGATATCCTTGAGCGGATGAAGGGTTCTGAAAATAAAAAAGAAAA
ACAGCGTGACCCACCGAAAACAGAAAAAAAGGATGAAACCAGCGGCCTCAGGTCCCTGATCGGGAAGAAGGATGGGCTCG
ACCTCAGCGCCCTCGTCCGTGACCCCCGTAATTATGGCCTGGGTGAGGAGAAACCCGCAGCACCTCCGGGCACAGAGGAA
CCGGCCGACACACCGATCGCTGAGACACCCCATCACGAGCCGCAGGACGAAGAATTCGACGATCAAGCCATCAGGGAACT
GAAATCAGCAGTCGACGCCATACTCAGTGCTCGGGAAGACGAAGCCCCTGCTCCGACTCTCTCCATGGAAGAAGAGACCC
CCCTGGAGGAGGAGGCCGTCTCTCCCGCTCCGGAGGCTGTGCCCGTCTCCAGAAAGAGCAGATGGTCCTCCGAAGTTGGG
AACCCTGAAGAGGAACCGGGCGCTGACATCACCGACGTCGACGACCTCAGTGTGTACTCCGACCTCATCCTCCCGAAGGC
TGCGACCTTCGATATCGAGGAGTTCACCCTCCAGCGACCCAACCACCGCGAGACCCTCGGCGTCAACAACCTCCCCCCTG
AGATTGACGTCCTCTGGGCCGACACGGTCCCGACGATTGCGGAAGAAGCCAGGAATGTTGTGATCGGCAGGGAAAAGGGG
GAAAAATCAGGCTTCCTCGGCAAGATCGGGGGGATCGGCAAGATAGGAGCCTTCGACCGGACCCAACACAAATCCAGTGT
CGAGGAGTATGACCCTGCGGTTCACGGTCCGCTCGTTGACCTCACCTTCACCCCCCCCGCCGGTGTCGAAGAGGTCGAAC
TCTACCCGGTCAACGAGCCCTTCGCCTATGTGCGGATATCCTATGACAGCACCTCTCACGAGTACAGTTACGAGGTGCTC
GAACCCGCCCTGACCGCAGCCGAGAACGAACTTTTCGGCGAGATCAAGGACCGGCTCTTCGAGAGACTCGACGTCAGCAC
CAAGGATCTCTCGCGGGAAGGGGCACGGCAGATCCTGCGCGACCTCTCCGACGAGATCGTCGCCGACTACGGGATCCATC
TCTCCTCCCTCTCCAGGGAGAAGATCTACTATACCATTGAACGTGACTTCCTCGGCAACGGTCTGATCGATGCGATCATG
CACGACAAGTACATCGAGGATATCTCCTGCGACGGACTGGACAACCCGGTCTTTGTCTTCCACTCTGGCTATGAGTCGAT
CAAGACGAACCTGATGTACACCAACGCCGTTGAACTCGATTCTTTCGTGACCAAACTTGCCCAGCGGGCTGGAAAATATA
TCTCCATCGCTGATCCGATCCTGGACGCCACGATGTCGGACGGTTCTCGTATCCAGATGACGTTAGGCAAGGAAGTGACC
GCTCACGGCTCGACCTTCACCATCAGGAAATTCAAGGACGAACCGATCACCCCGACCGACCTCATCGGCTGGGGCACCTT
CTCCCCCCTCTCGGTCGCCTTCCTCTGGCTTGCGGTCGAGGCCGGAAACTCGGCGATCTTTGCGGGTGGGACGGCTTCGG
GCAAGACCACCGCCCTCAATGCCATCTCACTCTTCATCCCGCCGCAGGCCAAGATCGTCTCCCTCGAGGACACCAGGGAA
CTGAAACTCCCGCACGCCAACTGGATCCCCTCGGTGACCAGGGAGTCCTTCGACACCGAAGGCAAAGGTACGATCGACAT
GTACGAACTCCTCCGTGCGGCCCTCCGACAGCGGCCCGAGTACATGTGTGTGGGCGAGGTCAGAGGTGCGGAGGCCCAGA
CCCTCTTCCAGGCCATGTCCACCGGCCACGTCACCTATGCCACCATGCACGCCGACTCGGTCGCAAGTGTCGTCCACCGT
CTCGAAAACCCACCGCTGGATGTGCCGCGTAACATGCTCAACGCTCTCAACCTCGTTTGTATCCAGGCTCAGGCCAGGAT
CGGCGGGCAGCGGATCAGGCGGAACAAACAGATCATCGAGATCCTGGACATTGACCCAAGGACCAACGAACTGATCACCA
ACGAGGTCTTCTCCTGGCACCAGGCCACCGACGAGATCCGGTACTCGGGCAAGTCGTACATCCTTGAGACGATCATGGAG
ACGAAGGGTTGGAGCGAGGCCAGGATGCGTGAAGAACTGAAGAGACGCCAGGAGATGCTTGAGTGGATGCGGGTCAAGAA
CATCAGACACTACAAGGATGTTTCCAAGATGCTTATCTCCTACTTCCGGGACGCCGATGCAGTAATCGGGCTTGTGCGGG
AAGAACTCTACAGGGAGGCTGATCAACAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(397-653)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8A3S4B9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdL Methanoculleus thermophilus strain DSM 2373

66.301

81.481

0.54

Multiple sequence alignment