Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   GII89_RS08655 Genome accession   NZ_CP045812
Coordinates   1659400..1661448 (+) Length   682 a.a.
NCBI ID   WP_003244692.1    Uniprot ID   O34942
Organism   Bacillus subtilis strain P8_B3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1654400..1666448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII89_RS08625 (GII89_08625) spoVM 1655036..1655116 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  GII89_RS08630 (GII89_08630) rpmB 1655189..1655377 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  GII89_RS08635 (GII89_08635) yloU 1655654..1656016 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  GII89_RS08640 (GII89_08640) fakA 1656032..1657693 (+) 1662 WP_003245033.1 DAK2 domain-containing protein -
  GII89_RS08645 (GII89_08645) sdaAB 1657832..1658494 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  GII89_RS08650 (GII89_08650) sdaAA 1658520..1659422 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  GII89_RS08655 (GII89_08655) recG 1659400..1661448 (+) 2049 WP_003244692.1 ATP-dependent DNA helicase RecG Machinery gene
  GII89_RS08660 (GII89_08660) fapR 1661557..1662123 (+) 567 WP_003232044.1 transcription factor FapR -
  GII89_RS08665 (GII89_08665) plsX 1662137..1663138 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  GII89_RS08670 (GII89_08670) fabD 1663157..1664110 (+) 954 WP_003245314.1 ACP S-malonyltransferase -
  GII89_RS08675 (GII89_08675) fabG 1664103..1664843 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  GII89_RS08680 (GII89_08680) acpP 1664927..1665160 (+) 234 WP_003154310.1 acyl carrier protein -
  GII89_RS08685 (GII89_08685) rncS 1665300..1666049 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78140.72 Da        Isoelectric Point: 7.4260

>NTDB_id=346350 GII89_RS08655 WP_003244692.1 1659400..1661448(+) (recG) [Bacillus subtilis strain P8_B3]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=346350 GII89_RS08655 WP_003244692.1 1659400..1661448(+) (recG) [Bacillus subtilis strain P8_B3]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34942

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

100

100

1
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38