Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW38_RS20935 Genome accession   NZ_CP036026
Coordinates   4032427..4033470 (-) Length   347 a.a.
NCBI ID   WP_016106153.1    Uniprot ID   A0A1G4EMB3
Organism   Bacillus mycoides strain BPN54/2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4027427..4038470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW38_RS20890 (EXW38_21205) - 4027825..4028025 (-) 201 WP_002015009.1 YqzE family protein -
  EXW38_RS20895 (EXW38_21210) - 4028064..4028561 (-) 498 WP_002160495.1 shikimate kinase -
  EXW38_RS20900 (EXW38_21215) - 4028714..4029364 (-) 651 WP_016106149.1 2OG-Fe(II) oxygenase -
  EXW38_RS20905 (EXW38_21220) comGG 4029538..4029909 (-) 372 WP_016106150.1 competence type IV pilus minor pilin ComGG -
  EXW38_RS20910 (EXW38_21225) comGF 4029906..4030367 (-) 462 WP_078181634.1 competence type IV pilus minor pilin ComGF -
  EXW38_RS20915 (EXW38_21230) - 4030351..4030647 (-) 297 WP_002160491.1 hypothetical protein -
  EXW38_RS20920 (EXW38_21235) comGD 4030640..4031095 (-) 456 WP_002160490.1 competence type IV pilus minor pilin ComGD -
  EXW38_RS20925 (EXW38_21240) comGC 4031092..4031391 (-) 300 WP_002160489.1 competence type IV pilus major pilin ComGC -
  EXW38_RS20930 (EXW38_21245) comGB 4031403..4032440 (-) 1038 WP_078172815.1 competence type IV pilus assembly protein ComGB -
  EXW38_RS20935 (EXW38_21250) comGA 4032427..4033470 (-) 1044 WP_016106153.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW38_RS20940 (EXW38_21255) - 4033676..4034371 (+) 696 WP_078172814.1 helix-turn-helix transcriptional regulator -
  EXW38_RS20945 (EXW38_21260) - 4034504..4034746 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  EXW38_RS20950 (EXW38_21265) - 4034854..4036248 (+) 1395 WP_215572041.1 L-cystine transporter -
  EXW38_RS31140 (EXW38_21270) - 4036498..4036766 (-) 269 Protein_4117 hypothetical protein -
  EXW38_RS20955 (EXW38_21275) - 4036767..4037018 (-) 252 WP_215572042.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39141.40 Da        Isoelectric Point: 8.4517

>NTDB_id=346040 EXW38_RS20935 WP_016106153.1 4032427..4033470(-) (comGA) [Bacillus mycoides strain BPN54/2]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGETRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346040 EXW38_RS20935 WP_016106153.1 4032427..4033470(-) (comGA) [Bacillus mycoides strain BPN54/2]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAAAGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTAGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCGTTGTCTCATCTTTCGTTATTTCCAAGTACAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACGACAATGTATGCGTTATTAGAAGTA
GCAAGAAAAGGAGAAACACGTCGCATCGTTACGCTGGAAGATCCAGTTGAGAGAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATGAAACGGGATTAAAGGCTATTTTGCGTCACGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTGATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGACTCGTTGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGGCAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTGCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G4EMB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Haemophilus influenzae 86-028NP

38.82

92.795

0.36
  pilB Glaesserella parasuis strain SC1401

43.706

82.421

0.36

Multiple sequence alignment