Detailed information NTG0346039

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: GH799_RS01420
• Genome accession: NZ_CP045794
• Coordinates: 292260..294464 (+)
• Length: 734 a.a.
• NCBI ID: WP_005496931.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus 10329
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 287260..299464

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GH799_RS01375 (GH799_01375)	rplV	287929..288261 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
GH799_RS01380 (GH799_01380)	rpsS	288272..288550 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
GH799_RS01385 (GH799_01385)	rplB	288572..289396 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
GH799_RS01390 (GH799_01390)	rplW	289412..289714 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
GH799_RS01395 (GH799_01395)	rplD	289711..290313 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
GH799_RS01400 (GH799_01400)	rplC	290331..290960 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
GH799_RS01405 (GH799_01405)	rpsJ	290975..291286 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
GH799_RS01410 (GH799_01410)	rpmE	291744..291965 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
GH799_RS01420 (GH799_01420)	priA	292260..294464 (+)	2205	WP_005496931.1	primosomal protein N'	Machinery gene
GH799_RS01425 (GH799_01425)	cytR	294758..295804 (+)	1047	WP_254894148.1	DNA-binding transcriptional regulator CytR	Regulator
GH799_RS01430 (GH799_01430)	ftsN	295980..296525 (+)	546	WP_005496929.1	cell division protein FtsN	-
GH799_RS01435 (GH799_01435)	hslV	296689..297240 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
GH799_RS01440 (GH799_01440)	hslU	297264..298595 (+)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82275.86 Da
• Isoelectric Point: 8.6765

>NTDB_id=346039 GH799_RS01420 WP_005496931.1 292260..294464(+) (priA) [Vibrio parahaemolyticus 10329]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWSEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALESIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=346039 GH799_RS01420 WP_005496931.1 292260..294464(+) (priA) [Vibrio parahaemolyticus 10329]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGTATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCCAAAGATCAACTCAAGCCAATCAAAGCGGTTCTTGATTCTCAGCCGGTATGGTCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAATTCTACCAGTACCCACTTGGAGATACGCTACACAATGCGATGCCGGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAGTCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAGTTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
ATGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTCATCCACTCCGGGTTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTA
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGTTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGCACGCGCCGAAAAGGCAGCTTAGAAGACGCGTTGGAATCCATCCGTAAGGGTGAAT
ATCAAATTTTGATTGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTACAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAAG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGACTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCACACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.284	100	0.435
priA	Bacillus subtilis subsp. subtilis str. 168	34.131	100	0.369