Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GHR40_RS08420 Genome accession   NZ_CP045741
Coordinates   1763303..1763962 (-) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain DH5alpha     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1758303..1768962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHR40_RS08395 (GHR40_08395) parE 1758394..1760286 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  GHR40_RS08400 (GHR40_08400) ygiN 1760334..1760648 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  GHR40_RS08405 (GHR40_08405) mdaB 1760679..1761260 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GHR40_RS08410 (GHR40_08410) ygiZ 1761579..1761911 (+) 333 WP_000917684.1 DUF2645 family protein -
  GHR40_RS08415 (GHR40_08415) qseC 1761957..1763306 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  GHR40_RS08420 (GHR40_08420) ciaR 1763303..1763962 (-) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  GHR40_RS08425 (GHR40_08425) ygiW 1764114..1764506 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GHR40_RS08430 (GHR40_08430) ygiV 1764559..1765041 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  GHR40_RS08435 (GHR40_08435) mqsR 1765246..1765542 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  GHR40_RS08440 (GHR40_08440) mqsA 1765544..1765939 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  GHR40_RS08445 (GHR40_08445) ygiS 1766072..1767679 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=345799 GHR40_RS08420 WP_001221495.1 1763303..1763962(-) (ciaR) [Escherichia coli strain DH5alpha]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=345799 GHR40_RS08420 WP_001221495.1 1763303..1763962(-) (ciaR) [Escherichia coli strain DH5alpha]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361