Detailed information NTG0345664

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EXW63_RS08425
• Genome accession: NZ_CP035997
• Coordinates: 1641284..1645009 (-)
• Length: 1241 a.a.
• NCBI ID: WP_215552769.1
• Uniprot ID: -
• Organism: Bacillus mycoides strain BPN36/3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1636284..1650009

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW63_RS08375 (EXW63_08550)	-	1636602..1637504 (-)	903	WP_002140809.1	fumarylacetoacetate hydrolase family protein	-
EXW63_RS08380 (EXW63_08555)	-	1637748..1637927 (+)	180	WP_215552768.1	aspartyl-phosphate phosphatase Spo0E family protein	-
EXW63_RS08385 (EXW63_08560)	gerPA	1638024..1638245 (+)	222	WP_001111190.1	spore germination protein GerPA	-
EXW63_RS08390 (EXW63_08565)	gerPB	1638260..1638466 (+)	207	WP_001012505.1	spore germination protein GerPB	-
EXW63_RS08395 (EXW63_08570)	gerPC	1638534..1639148 (+)	615	WP_001070757.1	spore germination protein GerPC	-
EXW63_RS08400 (EXW63_08575)	gerPD	1639155..1639349 (+)	195	WP_001052805.1	spore germination protein GerPD	-
EXW63_RS08405 (EXW63_08580)	-	1639364..1639750 (+)	387	WP_002030295.1	spore germination protein GerPE	-
EXW63_RS08410 (EXW63_08585)	gerPF	1639793..1640008 (+)	216	WP_001141570.1	spore germination protein GerPF	-
EXW63_RS08415 (EXW63_08590)	-	1640059..1640766 (-)	708	WP_002030297.1	DNA alkylation repair protein	-
EXW63_RS08420 (EXW63_08595)	-	1640982..1641260 (-)	279	WP_002011271.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
EXW63_RS08425 (EXW63_08600)	addA	1641284..1645009 (-)	3726	WP_215552769.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EXW63_RS08430 (EXW63_08605)	addB	1645006..1648521 (-)	3516	WP_215552770.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EXW63_RS08435 (EXW63_08610)	lepB	1648639..1649202 (-)	564	WP_215552771.1	signal peptidase I	-
EXW63_RS08440 (EXW63_08615)	-	1649259..1649852 (-)	594	WP_002011278.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142556.84 Da
• Isoelectric Point: 4.9396

>NTDB_id=345664 EXW63_RS08425 WP_215552769.1 1641284..1645009(-) (addA) [Bacillus mycoides strain BPN36/3]
MKENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKLLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEKHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEGGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQEGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYSASWK
VEVVEGKDLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYADATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIDE

Nucleotide

• Length: 3726 bp

>NTDB_id=345664 EXW63_RS08425 WP_215552769.1 1641284..1645009(-) (addA) [Bacillus mycoides strain BPN36/3]
ATGAAAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCGGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAATTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCCATTTCAACAATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAACTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAACCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGACGTTGAAGGAA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAGTTTCAGCTTGAAACAGCGGAAAAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGCGTTGAAACTTTACAAGCAGATTTAGCTTTACT
TGGAACGTTATCCTCAGCAGCGCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAAAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCCGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGGCGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCGGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGCGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACTGAGGAAGAAGTGCAAGAAGGTGAAGAAGGTGCCGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCAATGGTCGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCGGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAATGTATTCCGCGTCATTGATAAC
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTCGCGACGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAGGGAGCGCCGCTTGAAGAAGAACAAGAAT
TACATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGGTGGCGTGAATTTGCGCGACAACAATCACTTTCCGATTTA
ATTTGGAAAGTGTATGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGATCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAGC
GTATTTTAGAACGCGGAGATGATATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACAGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATTCGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAGGCGAAATTTATGAATATAGTGCTAGCTGGAAA
GTAGAAGTTGTTGAAGGAAAAGATTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
TGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGGAATACGGGTATGCGG
ACGCGACATCTCACCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAGCGCGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATATTTCTTTGATGGAAATCATGTTGTAAATATTGACGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.569	100	0.538