Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   GHB96_RS02315 Genome accession   NZ_CP045707
Coordinates   448653..449588 (+) Length   311 a.a.
NCBI ID   WP_215772541.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG250     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443653..454588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS02290 (GHB96_02410) - 444146..444655 (+) 510 WP_003687909.1 isoprenylcysteine carboxyl methyltransferase family protein -
  GHB96_RS02295 (GHB96_02415) - 444896..445477 (-) 582 WP_003698180.1 superoxide dismutase -
  GHB96_RS02300 (GHB96_02420) dnaB 445640..447046 (+) 1407 WP_215772538.1 replicative DNA helicase -
  GHB96_RS02305 (GHB96_02425) pilH 447354..448016 (+) 663 WP_215772539.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  GHB96_RS02310 (GHB96_02430) pilV/pilI 448048..448656 (+) 609 WP_215772540.1 type IV pilus modification protein PilV Regulator
  GHB96_RS02315 (GHB96_02435) pilJ 448653..449588 (+) 936 WP_215772541.1 PilW family protein Machinery gene
  GHB96_RS02320 (GHB96_02440) pilK 449567..450166 (+) 600 WP_050157381.1 pilus assembly protein Machinery gene
  GHB96_RS02325 (GHB96_02445) pilL 450168..450641 (+) 474 WP_215772556.1 PilX family type IV pilin Machinery gene
  GHB96_RS02330 (GHB96_02450) - 450711..451019 (-) 309 WP_025456241.1 AzlD family protein -
  GHB96_RS02335 (GHB96_02455) - 451016..451727 (-) 712 Protein_459 AzlC family ABC transporter permease -
  GHB96_RS02340 (GHB96_02460) dut 451893..452345 (+) 453 WP_003687923.1 dUTP diphosphatase -
  GHB96_RS02345 (GHB96_02465) dapC 452423..453610 (+) 1188 WP_003687924.1 succinyldiaminopimelate transaminase -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 34280.23 Da        Isoelectric Point: 9.1731

>NTDB_id=345372 GHB96_RS02315 WP_215772541.1 448653..449588(+) (pilJ) [Neisseria gonorrhoeae strain NG250]
MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSIIVLIAVVSSYFTSRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFG
CFNMSEHIKDVVVSDVAQKNYLFLLKKSNANKLIPIAESLNINYRGFLQVGSALIFQYGIDDVNASADTTVVSSCAVISK
PGKQILNLEDVKKELKIASQDKEQNGNIARQRHVVNAYAVGRFGNEEGLFRFQLNEKGEWGNPQLLAKKIKRMRVRYIYV
SGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVLLDSGGSDAKIAASSDNIIYAYRINATIRGGNVCANRTL

Nucleotide


Download         Length: 936 bp        

>NTDB_id=345372 GHB96_RS02315 WP_215772541.1 448653..449588(+) (pilJ) [Neisseria gonorrhoeae strain NG250]
ATGAAACGTAAAATGCTAAACGTACCAAAGGGCGGTTATGATGGTATGAAGGGTTTTACCATTGTTGAATTTCTGGTTGC
GGGCCTGCTCAGTATAATTGTCCTGATAGCGGTCGTATCGAGTTACTTTACATCCCGGAAATTAAATGATGCGGCAAACG
AGCGTCTTGCCGAGCAACAGGATTTGCGGAATGCGGCAACATTGATTGTCCGCGATGCAAGAATGGCGGGGAGCTTCGGT
TGCTTCAATATGTCTGAGCATATCAAAGATGTTGTTGTTTCCGATGTGGCGCAAAAAAACTATCTTTTTTTGTTAAAAAA
GAGCAATGCAAATAAACTTATCCCCATAGCGGAATCTTTAAATATCAATTATCGGGGTTTTCTCCAGGTTGGTAGCGCAT
TGATTTTTCAATACGGAATCGATGATGTTAATGCAAGTGCCGATACTACCGTCGTCAGCAGCTGTGCCGTAATATCGAAA
CCGGGCAAGCAAATCCTTAATTTAGAAGATGTAAAAAAAGAATTGAAGATTGCGAGTCAGGATAAGGAGCAAAATGGCAA
TATAGCGCGTCAAAGGCATGTGGTCAATGCCTATGCGGTCGGCAGGTTTGGCAATGAGGAAGGTTTGTTCCGCTTCCAAT
TGAATGAAAAGGGCGAGTGGGGTAATCCTCAGTTGCTCGCGAAAAAGATTAAACGTATGAGAGTGCGGTATATCTATGTT
TCCGGTTGTCCTGAAGATGAAGATGCCGGCAAAGAGGAACAATTCAAATATACGGATAAATTCGACAGCTCTGTTACGCC
TGCCGGGGTGGAGGTTTTATTGGATAGCGGCGGTAGTGATGCCAAGATTGCCGCTTCTTCAGATAATATTATTTATGCTT
ACCGTATCAATGCGACAATACGCGGGGGAAATGTATGCGCAAACAGAACACTTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Neisseria gonorrhoeae MS11

87.58

100

0.884

Multiple sequence alignment