Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   GHB96_RS02305 Genome accession   NZ_CP045707
Coordinates   447354..448016 (+) Length   220 a.a.
NCBI ID   WP_215772539.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain NG250     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442354..453016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS02280 (GHB96_02400) cysT 442466..443302 (-) 837 WP_047923822.1 sulfate ABC transporter permease subunit CysT -
  GHB96_RS02285 (GHB96_02405) - 443483..443815 (+) 333 WP_003687908.1 hypothetical protein -
  GHB96_RS02290 (GHB96_02410) - 444146..444655 (+) 510 WP_003687909.1 isoprenylcysteine carboxyl methyltransferase family protein -
  GHB96_RS02295 (GHB96_02415) - 444896..445477 (-) 582 WP_003698180.1 superoxide dismutase -
  GHB96_RS02300 (GHB96_02420) dnaB 445640..447046 (+) 1407 WP_215772538.1 replicative DNA helicase -
  GHB96_RS02305 (GHB96_02425) pilH 447354..448016 (+) 663 WP_215772539.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  GHB96_RS02310 (GHB96_02430) pilV/pilI 448048..448656 (+) 609 WP_215772540.1 type IV pilus modification protein PilV Regulator
  GHB96_RS02315 (GHB96_02435) pilJ 448653..449588 (+) 936 WP_215772541.1 PilW family protein Machinery gene
  GHB96_RS02320 (GHB96_02440) pilK 449567..450166 (+) 600 WP_050157381.1 pilus assembly protein Machinery gene
  GHB96_RS02325 (GHB96_02445) pilL 450168..450641 (+) 474 WP_215772556.1 PilX family type IV pilin Machinery gene
  GHB96_RS02330 (GHB96_02450) - 450711..451019 (-) 309 WP_025456241.1 AzlD family protein -
  GHB96_RS02335 (GHB96_02455) - 451016..451727 (-) 712 Protein_459 AzlC family ABC transporter permease -
  GHB96_RS02340 (GHB96_02460) dut 451893..452345 (+) 453 WP_003687923.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 220 a.a.        Molecular weight: 24397.95 Da        Isoelectric Point: 9.6051

>NTDB_id=345370 GHB96_RS02305 WP_215772539.1 447354..448016(+) (pilH) [Neisseria gonorrhoeae strain NG250]
MCTRKQQGFTLIELLIVMAIAAIMATIALPNMGGWIASRRIASHAEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGAPN
NGCDFSKKGRGMLAFGDKNGNKVYDGDIKDVFLRNVVLNDTDDSRINYAFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
QNLKDNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCRKNDTRAVCKH

Nucleotide


Download         Length: 663 bp        

>NTDB_id=345370 GHB96_RS02305 WP_215772539.1 447354..448016(+) (pilH) [Neisseria gonorrhoeae strain NG250]
ATGTGTACACGAAAACAACAAGGTTTCACGCTAATAGAGCTGCTCATCGTGATGGCCATTGCAGCCATTATGGCGACGAT
AGCCCTCCCCAATATGGGCGGGTGGATTGCATCACGCCGCATTGCCAGTCACGCGGAGCAGGTTGCCAACCTTTTGCGTT
TTTCCAGGGGCGAAGCCGTTCGGCTCAATCTCCCTGTCTATATCTGTCCTGTTCAAGTTAAAAAAGACGGTGCGCCCAAC
AATGGATGTGATTTCAGCAAGAAGGGGCGGGGAATGTTGGCTTTCGGCGACAAAAACGGCAATAAGGTATATGACGGCGA
TATTAAGGATGTTTTTCTCCGCAACGTGGTATTGAATGATACCGACGACAGCCGGATTAATTATGCCTTCAACCATATCG
CTTTCGGTTCGTCTCAGCCGAAAGCCGACCGTGTAGTTTGGACGTTCAACCAAAACGGGACATTCGGCTATTTGCCCGAT
CAGAATCTCAAGGATAATTCCAAATTTGTTTATTCCGACGGTTATATCCAAATCGTGCTGACAGATGCGAGAGCAGTTTC
AGATGCCGATAAGAAATTCCGTTCGGCGGTGGTTTTGATTGACAGCAGCGGCAGGGTCGAAGTTTGTCGTAAAAACGATA
CGCGCGCCGTATGCAAACATTAA

Domains

Predicted by InterProScan.

Type II transport protein GspH (GspH)

(44-204)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Neisseria gonorrhoeae MS11

95.455

100

0.955