Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   EXW58_RS00020 Genome accession   NZ_CP035953
Coordinates   3023..4150 (+) Length   375 a.a.
NCBI ID   WP_002159660.1    Uniprot ID   J8FJG8
Organism   Bacillus mycoides strain BPN03/1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW58_RS00005 (EXW58_00005) dnaA 1..1341 (+) 1341 WP_002144554.1 chromosomal replication initiator protein DnaA -
  EXW58_RS00010 (EXW58_00010) dnaN 1524..2669 (+) 1146 WP_002068698.1 DNA polymerase III subunit beta -
  EXW58_RS00015 (EXW58_00015) yaaA 2798..3010 (+) 213 WP_002159661.1 S4 domain-containing protein YaaA -
  EXW58_RS00020 (EXW58_00020) recF 3023..4150 (+) 1128 WP_002159660.1 DNA replication/repair protein RecF Machinery gene
  EXW58_RS00025 (EXW58_00025) gyrB 4189..6111 (+) 1923 WP_033710559.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  EXW58_RS00030 (EXW58_00030) gyrA 6200..8671 (+) 2472 WP_002159658.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43518.78 Da        Isoelectric Point: 6.9411

>NTDB_id=345248 EXW58_RS00020 WP_002159660.1 3023..4150(+) (recF) [Bacillus mycoides strain BPN03/1]
MFITEIQLKNYRNYEHLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGNIKGRLQRRNSS
LSLELNISKKGKKAKLNHLEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGAKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLLGPHRDDLHFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=345248 EXW58_RS00020 WP_002159660.1 3023..4150(+) (recF) [Bacillus mycoides strain BPN03/1]
TTGTTTATTACAGAGATACAATTAAAAAACTATCGCAATTATGAGCATTTAGAGCTTTCCTTTGAGGATAAAGTCAATGT
AATTATTGGTGAAAATGCGCAAGGGAAAACGAATCTGATGGAAGCTATTTATGTATTGGCGATGGCGAAATCCCATAGAA
CTTCGAACGATCGCGAACTCATTCGTTGGGATGAGGATTATGGTAATATAAAAGGTAGATTACAAAGGCGAAATAGTTCA
TTATCCTTAGAATTAAATATTTCAAAAAAAGGTAAAAAGGCAAAGTTAAATCACTTAGAACAACAAAAATTGAGCCAGTA
TATTGGTGAAATGAATGTTGTTATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCCCAAGTACGAAGACGCT
TTTTGGATATGGAACTTGGACAAATAGCTCCGGTCTATTTGTATGAATTAAGTCAATATCAAAAGGTACTCACGCAACGA
AATCATTTACTTAAGAAGATGCAAGGGAATAGTAAAAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTGCAAAAATTTTGCGAAAACGTTTTGAGTTTTTGCATTTACTACAAGAATGGGCTGCTCCAATTCACCGCGGGA
TTAGCAGGGGATTAGAGGAATTAGAAATTGTTTATAAACCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTTCGTGGTACAACTTTACTAGGTCCTCATCG
TGATGATTTACACTTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCACAAGGACAACAACGAACAACCGCACTAT
CCCTAAAATTGGCTGAAATTGAATTGATATACTCAGAGGTTAAGGAATATCCAATCCTTTTACTAGATGATGTGTTATCA
GAATTAGATGATTATCGCCAATCGCATCTGCTAAATACAATTCAAGGAAAAGTACAAACCTTTGTGACGACGACGAGTGT
CGACGGAATTGAACACGAAACATTGAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGACA
GGAAATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8FJG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

67.391

98.133

0.661

Multiple sequence alignment