Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EPB55_RS07910 Genome accession   NZ_CP035731
Coordinates   1562866..1563456 (-) Length   196 a.a.
NCBI ID   WP_011079354.1    Uniprot ID   A0A3Q0L3M6
Organism   Vibrio vulnificus strain 2142-77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1557866..1568456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB55_RS07890 aroB 1558721..1559813 (-) 1093 Protein_1490 3-dehydroquinate synthase -
  EPB55_RS07895 aroK 1559846..1560364 (-) 519 WP_011079357.1 shikimate kinase AroK -
  EPB55_RS07900 pilQ 1560566..1562350 (-) 1785 WP_103277325.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB55_RS07905 pilP 1562364..1562876 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  EPB55_RS07910 pilO 1562866..1563456 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB55_RS07915 pilN 1563449..1564039 (-) 591 WP_045598453.1 PilN domain-containing protein Machinery gene
  EPB55_RS07920 pilM 1564029..1565036 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  EPB55_RS07925 - 1565176..1567689 (+) 2514 WP_103277324.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22749.25 Da        Isoelectric Point: 5.0836

>NTDB_id=344115 EPB55_RS07910 WP_011079354.1 1562866..1563456(-) (pilO) [Vibrio vulnificus strain 2142-77]
MASWQDLDIDEITDWPILPQAAVVVLLMLIIQGFGYWFYLKPETEVLQRMVTEEQTLKISARIKATKVAALPQLQSQLDE
LTQRYEFLLKQLPVQKELATMLASVNELGIQSKLTFTRIDWGEKQTKDFLYQLPLNIELTGDYHDIGEFSEAIARLPRII
SFTDVTWHRVSMESSTLHFRVKANTYQFKSEAVHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=344115 EPB55_RS07910 WP_011079354.1 1562866..1563456(-) (pilO) [Vibrio vulnificus strain 2142-77]
ATGGCTAGTTGGCAAGATCTCGATATTGATGAAATCACAGATTGGCCGATTTTACCGCAGGCGGCGGTTGTCGTGTTGCT
GATGTTGATCATTCAAGGTTTTGGTTATTGGTTCTATCTCAAGCCGGAAACGGAAGTGTTGCAAAGGATGGTCACAGAAG
AGCAAACGCTGAAAATCTCGGCTCGAATTAAGGCGACCAAAGTGGCTGCCTTGCCGCAACTTCAATCGCAACTTGATGAA
CTGACTCAGCGCTATGAATTCTTACTTAAGCAACTGCCCGTGCAGAAGGAGTTGGCGACGATGCTGGCCTCGGTCAATGA
ACTCGGCATTCAAAGTAAGCTCACGTTTACACGTATCGATTGGGGGGAGAAGCAAACCAAGGACTTCCTTTACCAACTGC
CTTTGAATATTGAGTTAACCGGGGATTATCACGATATCGGTGAATTTTCTGAAGCGATTGCCCGCTTGCCTCGCATTATC
AGTTTTACCGATGTGACTTGGCATCGAGTGAGTATGGAAAGCAGCACCTTGCACTTCCGAGTAAAAGCGAACACCTATCA
GTTCAAGTCGGAGGCTGTCCATGAGAAGTAG

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

73.016

96.429

0.704
  pilO Vibrio cholerae strain A1552

67.347

100

0.673

Multiple sequence alignment