Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB55_RS07920 Genome accession   NZ_CP035731
Coordinates   1564029..1565036 (-) Length   335 a.a.
NCBI ID   WP_011151279.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2142-77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559029..1570036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB55_RS07895 aroK 1559846..1560364 (-) 519 WP_011079357.1 shikimate kinase AroK -
  EPB55_RS07900 pilQ 1560566..1562350 (-) 1785 WP_103277325.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB55_RS07905 pilP 1562364..1562876 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  EPB55_RS07910 pilO 1562866..1563456 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB55_RS07915 pilN 1563449..1564039 (-) 591 WP_045598453.1 PilN domain-containing protein Machinery gene
  EPB55_RS07920 pilM 1564029..1565036 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  EPB55_RS07925 - 1565176..1567689 (+) 2514 WP_103277324.1 PBP1A family penicillin-binding protein -
  EPB55_RS07930 oxyR 1567756..1568655 (-) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  EPB55_RS07935 - 1568929..1569201 (+) 273 WP_103277323.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37638.05 Da        Isoelectric Point: 6.3873

>NTDB_id=344117 EPB55_RS07920 WP_011151279.1 1564029..1565036(-) (pilM) [Vibrio vulnificus strain 2142-77]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=344117 EPB55_RS07920 WP_011151279.1 1564029..1565036(-) (pilM) [Vibrio vulnificus strain 2142-77]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCGTTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.433

100

0.534
  pilM Vibrio cholerae strain A1552

49.267

100

0.501

Multiple sequence alignment