Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GBF15_RS03510 Genome accession   NZ_CP045277
Coordinates   724518..725177 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain LAU-OXA     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 719518..730177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBF15_RS03485 parE 719609..721501 (+) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -
  GBF15_RS03490 ygiN 721549..721863 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  GBF15_RS03495 mdaB 721894..722475 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  GBF15_RS03500 ygiZ 722794..723126 (+) 333 WP_000917684.1 DUF2645 family protein -
  GBF15_RS03505 qseC 723172..724521 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  GBF15_RS03510 ciaR 724518..725177 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  GBF15_RS03515 ygiW 725329..725721 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GBF15_RS03520 ygiV 725774..726256 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  GBF15_RS03525 mqsR 726461..726757 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  GBF15_RS03530 mqsA 726759..727154 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  GBF15_RS03535 ygiS 727287..728894 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=344069 GBF15_RS03510 WP_001221493.1 724518..725177(-) (ciaR) [Escherichia coli strain LAU-OXA]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=344069 GBF15_RS03510 WP_001221493.1 724518..725177(-) (ciaR) [Escherichia coli strain LAU-OXA]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365