Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GC231_RS20950 Genome accession   NZ_CP045213
Coordinates   4278933..4279592 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain EH41     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4273933..4284592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GC231_RS20930 (GC231_21090) plsC 4274080..4274817 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  GC231_RS20935 (GC231_21095) parC 4275050..4277308 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  GC231_RS20940 (GC231_21100) ygiV 4277854..4278336 (-) 483 WP_000183494.1 AraC family transcriptional regulator -
  GC231_RS20945 (GC231_21105) ygiW 4278389..4278781 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GC231_RS20950 (GC231_21110) ciaR 4278933..4279592 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  GC231_RS20955 (GC231_21115) qseC 4279589..4280938 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  GC231_RS20960 (GC231_21120) - 4280984..4281268 (-) 285 WP_097712940.1 DUF2645 family protein -
  GC231_RS20965 (GC231_21125) mdaB 4281635..4282216 (+) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  GC231_RS20970 (GC231_21130) ygiN 4282247..4282561 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  GC231_RS20975 (GC231_21135) - 4282605..4283936 (-) 1332 WP_000778880.1 MFS transporter -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=344039 GC231_RS20950 WP_001221502.1 4278933..4279592(+) (ciaR) [Escherichia coli strain EH41]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=344039 GC231_RS20950 WP_001221502.1 4278933..4279592(+) (ciaR) [Escherichia coli strain EH41]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment