Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EPB54_RS15645 Genome accession   NZ_CP035701
Coordinates   3108932..3110680 (-) Length   582 a.a.
NCBI ID   WP_005488227.1    Uniprot ID   A0A7H5D3I7
Organism   Vibrio parahaemolyticus strain 2012AW-0154     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103932..3115680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB54_RS15625 - 3104614..3105453 (-) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EPB54_RS15630 - 3105523..3107040 (-) 1518 WP_154119948.1 AAA family ATPase -
  EPB54_RS15635 aroB 3107093..3108193 (-) 1101 WP_025546399.1 3-dehydroquinate synthase -
  EPB54_RS15640 aroK 3108225..3108743 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EPB54_RS15645 pilQ 3108932..3110680 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB54_RS15650 pilP 3110712..3111227 (-) 516 WP_031778292.1 pilus assembly protein PilP Machinery gene
  EPB54_RS15655 pilO 3111217..3111810 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB54_RS15660 pilN 3111803..3112381 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  EPB54_RS15665 pilM 3112365..3113381 (-) 1017 WP_031778293.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63751.85 Da        Isoelectric Point: 4.4353

>NTDB_id=343971 EPB54_RS15645 WP_005488227.1 3108932..3110680(-) (pilQ) [Vibrio parahaemolyticus strain 2012AW-0154]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGDGVGIDDFLNVNLAATNPNA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=343971 EPB54_RS15645 WP_005488227.1 3108932..3110680(-) (pilQ) [Vibrio parahaemolyticus strain 2012AW-0154]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGTATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAGCAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTTGAAGTGTT
CCGCGATACCTCCACCACAAGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTAGTGGTTTC
GGACTCTGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCCCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAGCTGACGGAAGAAATGGGTGAACTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATTAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAGTGAGGGTAACATGGATGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTTCTTAATGTGAATTTAGCCGCGACCAACCCGAATGCG
GCGAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCCGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGTACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTTCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGCAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D3I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.924

100

0.919
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Legionella pneumophila strain ERS1305867

41.016

94.674

0.388
  pilQ Legionella pneumophila strain Lp02

41.016

94.674

0.388
  pilQ Pseudomonas aeruginosa PAK

40.468

95.533

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment