Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GBG22_RS01255 Genome accession   NZ_CP045206
Coordinates   274726..276222 (+) Length   498 a.a.
NCBI ID   WP_001040644.1    Uniprot ID   B7MDV4
Organism   Escherichia coli strain DE17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269726..281222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBG22_RS01225 (GBG22_01265) tusA 269806..270051 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  GBG22_RS01230 (GBG22_01270) zntA 270320..272518 (-) 2199 WP_000106588.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GBG22_RS01235 (GBG22_01275) yhhN 272592..273218 (-) 627 WP_000964729.1 lysoplasmalogenase -
  GBG22_RS01240 (GBG22_01280) yhhM 273359..273718 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  GBG22_RS01245 (GBG22_01285) yhhL 273721..273990 (-) 270 WP_001295207.1 DUF1145 family protein -
  GBG22_RS01250 (GBG22_01290) rsmD 273980..274576 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GBG22_RS01255 (GBG22_01295) pilA 274726..276222 (+) 1497 WP_001040644.1 signal recognition particle-docking protein FtsY Machinery gene
  GBG22_RS01260 (GBG22_01300) ftsE 276225..276893 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GBG22_RS01265 (GBG22_01305) ftsX 276886..277944 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  GBG22_RS01270 (GBG22_01310) rpoH 278189..279043 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GBG22_RS01275 (GBG22_01315) dapA 279147..280031 (-) 885 WP_000661262.1 4-hydroxy-tetrahydrodipicolinate synthase -
  GBG22_RS01280 (GBG22_01320) - 280031..280951 (-) 921 WP_001332164.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54627.41 Da        Isoelectric Point: 4.1849

>NTDB_id=343817 GBG22_RS01255 WP_001040644.1 274726..276222(+) (pilA) [Escherichia coli strain DE17]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAANVVEVTEQVAE
SEKAQPEAEVVAQPESVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNGVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=343817 GBG22_RS01255 WP_001040644.1 274726..276222(+) (pilA) [Escherichia coli strain DE17]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCAACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAATCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGGGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7MDV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

44.667

90.361

0.404

Multiple sequence alignment